製品の概要

  • 製品名
    Anti-Apolipoprotein CI antibody
    Apolipoprotein CI 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Apolipoprotein CI
  • 由来種
    Goat
  • 特異性
    Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-I and has negligible cross-reactivity with Type A-I, A-II, B, C-II, C-III, E and J apoLipoproteins.
  • アプリケーション
    適用あり: Dot blot, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length synthetic apoLipoprotein Type C-I (purified).

  • 特記事項
    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/5000 - 1/10000.
ELISA 1/4000 - 1/8000.
WB 1/1000.

ターゲット情報

  • 機能
    Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • 組織特異性
    Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • 配列類似性
    Belongs to the apolipoprotein C1 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APO C1 antibody
    • Apo CI antibody
    • Apo-CIB antibody
    • Apo-CIB' antibody
    • APOC 1 antibody
    • ApoC I antibody
    • ApoC-IB antibody
    • ApoC-IB' antibody
    • APOC1 antibody
    • APOC1_HUMAN antibody
    • APOC1B antibody
    • Apolipoprotein C I antibody
    • Apolipoprotein C I variant I antibody
    • Apolipoprotein C-I antibody
    • Apolipoprotein C1 antibody
    • Apolipoprotein CI antibody
    • ApolipoproteinC I antibody
    • ApolipoproteinCI antibody
    • Truncated apolipoprotein C-I antibody
    see all

プロトコール

参考文献

ab7617 has not yet been referenced specifically in any publications.

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your phone call. I enquired with the originator of this antibody and it turns out that ab7617 has not yet been tested for application in IHC. There was an error on the datasheet which I have updated and I apologize for any confusion. This doesn't mean that the antibody won't work in IHC, just that it has not yet been tested. If you have already purchased the antibody, I can certainly offer a credit note or refund. Please contact us again if you have any additional questions.

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Answer

Thank you for your enquiry. The Apo C1 is purified from Human plasma by density gradient ultracentrifugation and HPLC. The protocol of the purification is proprietary. A general WB protocol is followed. This product has been assayed against 1.0 µg of Apo C1 in a standard indirect ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and ABTS as a substrate for 30 minutes at room temperature. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. The antibody was tested in WB (6.6kDa) and Elisa. The sample tested against was purified Human apoC1 protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I have received an inquiry about #ab7617. I send you Questionnaire sheet. Please answer for following question; 1. Order details: Batch number:ab7617, Lot.#81476 Abcam order or Purchase order number: Antibody storage conditions (temperature/reconstitution etc): Stored at 4 oC 2. Please describe the problem (high background, wrong band size, more bands, no band etc): No band were observed, however Apolipoprotein C-1 which was purified from HDL were loaded as a positive control. 3. On what material are you testing the antibody in WB? Species: Human Cell extract or Nuclear extract: Purified protein or Recombinant protein: Purified protein Apo C-1 (Sigma #A7785), Serum and Tissue (lien) 3. The lysate How much protein was loaded: 20 or 30 ug What lysis buffer was used: What protease inhibitors were used: What loading buffer was used: Loading buffer contains either 2-Mercaptoethanol or DTT Did you heat the samples: temperature and time: 5min for 100 in the presence of 2-ME, or room temperature in the presence of DTT 4. Electrophoresis/Gel conditions/ Transfer conditions Reducing or non reducing gel: Bio-Rad Ready gel J (Acrylamide Gel for protein or peptide Electrophoresis) Gel percentage: Upper gel: 4%, Lower gel: 16.5% or 10~20% Gradient gel Transfer conditions: 400mA 3hr or 10V 15hr 5. Blocking conditions Buffer: Tris/Glycine/SDS or Tris/Trycine/SDS Blocking agent: milk, BSA, serum, what percentage: 5% skim milk Incubation time: 1hr Incubation temperature: room temperature 6. Primary Antibody Specification (in which species was it raised against): goat At what dilution(s) have you tested this antibody: 1/200, 1/500, 1/1000 What dilution buffer was used: 5% skim milk Incubation time: 2hr/3hr Incubation temperature: room temperature What washing steps were done: 5% skim milk 7. Secondary Antibody Specification (in which species was it raised against)?: Goat IgG At what dilution(s) have you tested this antibody: 1/500 Incubation time: 1 or 5 hr Wash steps: 5% skim milk Do you know whether the problems you are experiencing come from the secondary?: 8. Detection method ECl, ECl+, other detection method: ECL 9. Background bands Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): goat beta-actin antibody was used primary control and its signal was detected. Is the blocking step sufficient?: Yes Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps): At what size are the bands migrating? Could they be degradation products of your target?: Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient): 11. Did you apply positive and negative controls along with the samples? Please specify.: Apolipoprotein C-1 (SIGMA, A7785, 100ug, 121K1395) was used as positive control. 10. Optimization attempts How many times have you tried the Western?: Do you obtain the same results every time e.g. are background bands always in the same place?: beta-actin is detected in normal position. The protein was detected on membrane by CBB staining, therefore transfer step work well. What steps have you altered?: WB was performed at various concentration of primary antibody.:

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Answer

I'm sorry to hear you are having problems with ab7617 in WB. I would like to recommend incubating the primary antibody overnight at 4C to promote binding of the antibody to the protein and using ECL+ as a detection kit as this is more sensitive than ECL. Please also consider decreasing the amount of milk used (incubate the antibodies in TBST only, no milk). Please let me know if you still have problems following these changes and I can arrange for a replacement vial to be sent to you if you purchased the antibody in the last 90 days.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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