Anti-Apolipoprotein B 抗体 (ab98132)
Key features and details
- Goat polyclonal to Apolipoprotein B
- Suitable for: IHC-P, WB, IP, ELISA, GSA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Apolipoprotein B antibody
Apolipoprotein B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Apolipoprotein B -
由来種
Goat -
特異性
This antibody has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins.
Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards.
Non-specific cross-reaction of anti-apolipoprotein antibodies with other Human serum proteins is negligible. -
アプリケーション
適用あり: IHC-P, WB, IP, ELISA, GSAmore details -
種交差性
交差種: Human -
免疫原
Full length native Human Apolipoprotein B isolated from Human plasma.
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ポジティブ･コントロール
- Human liver tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.435% Sodium chloride, 0.146% EDTA, 4.7625% Sodium borate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified by density gradient centrifugation, followed by HPLC purification -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab98132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| GSA |
Use at an assay dependent concentration.
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| EMSA |
Use at an assay dependent concentration.
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| 特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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GSA
Use at an assay dependent concentration. |
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EMSA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
関連疾患
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
配列類似性
Contains 1 vitellogenin domain. -
翻訳後修飾
Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
細胞内局在
Cytoplasm. Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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別名
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (ab98132)Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelled with ab98132 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (ab98132)This image is courtesy of an anonymous Abreviewab98132 staining Apolipoprotein B in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (0.5 ug/ml in BSA/TBS buffer) for 30 minutes. ab97101 Rabbit polyclonal Secondary Antibody to Anti-goat HRP, IgG - H&L, pre-adsorbed (1/500) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab98132 は 4 報の論文で使用されています。
- Giesecke Y et al. Developing Electron Microscopy Tools for Profiling Plasma Lipoproteins Using Methyl Cellulose Embedment, Machine Learning and Immunodetection of Apolipoprotein B and Apolipoprotein(a). Int J Mol Sci 21:N/A (2020). PubMed: 32887372
- Langevin SM et al. Balancing yield, purity and practicality: a modified differential ultracentrifugation protocol for efficient isolation of small extracellular vesicles from human serum. RNA Biol 16:5-12 (2019). PubMed: 30604646
- Chinen AB et al. The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids. Small 13:N/A (2017). PubMed: 28196309
- Podjaski C et al. Netrin 1 regulates blood-brain barrier function and neuroinflammation. Brain 138:1598-612 (2015). WB . PubMed: 25903786