Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Liver
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Diluent: 1% BSA in TBS
Dilution
1/50
Additional data
Additional Notes
The secondary was followed-up with Envision Rabbit + tertiary HRP from Dako.
Antibody shows no cross-reactivity with mouse ApoB. Perfect for our research.
Abcam response
Thank you for participating in the Abtrial program and your valuable feedback.
Please be aware that the customer was looking for an antibody that does not react with mouse.
To our knowledge, the antibody has not been experimentally tested in mouse before and we had no information about a possible cross-reaction. The results suggest that the antibody does not cross-react with mouse under those experimental conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 12 2015