Anti-APOLD1 抗体 (ab272654)

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Western blot - Anti-APOLD1 antibody (ab272654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOLD1 antibody (ab272654)
  • Immunocytochemistry/ Immunofluorescence - Anti-APOLD1 antibody (ab272654)

Key features and details

  • Rabbit polyclonal to APOLD1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-APOLD1 antibody

  • 製品の詳細

    Rabbit polyclonal to APOLD1

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human APOLD1 aa 100-200.
    Database link: Q96LR9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: RT4 and U-251 MG cells. IHC-P: Human placenta tissue. IF: SK-MEL-30 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
WB
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood-brain permeability.

  • 組織特異性

    Expressed in neonatal dermal microvascular endothelial cells.

  • 配列類似性

    Belongs to the apolipoprotein L family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • APLD1_HUMAN antibody
      • APOLD1 antibody
      • Apolipoprotein L domain containing protein 1 antibody
      • Apolipoprotein L domain-containing protein 1 antibody
      • Vascular early response gene protein antibody
      • VERGE antibody
      see all

    画像

    • Western blot - Anti-APOLD1 antibody (ab272654)
      Western blot - Anti-APOLD1 antibody (ab272654)
      Lanes 2-3 : Anti-APOLD1 antibody (ab272654) at 0.4 µg/ml

      Lane 1 : Molecular Weight ladder
      Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
      Lane 3 : U-251 MG cells (Human brain glioma cell line)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOLD1 antibody (ab272654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOLD1 antibody (ab272654)

      Paraffin-embedded formalin-fixed human placenta tissue stained for APOLD1 using ab272654 at 1/50 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-APOLD1 antibody (ab272654)
      Immunocytochemistry/ Immunofluorescence - Anti-APOLD1 antibody (ab272654)

      PFA fixed, Triton X-100 permeablized SK-MEL-30 cells (human cutaneous melanoma cell line) labeling APOLD1 (green) using ab272654 at 2 ug/ml in ICC/IF analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272654 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272654 は論文での使用が確認できていません。

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