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リコンビナントRabMAb

Anti-APOA4/Apo-AIV 抗体 [EPR2895] (ab108200)

Reviews (1) Submit a question References (1)
Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
  • Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
  • Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2895] to APOA4/Apo-AIV
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-APOA4/Apo-AIV antibody [EPR2895]
    APOA4/Apo-AIV 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2895] to APOA4/Apo-AIV

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human plasma

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108200の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP
1/10 - 1/100.
追加情報
Is unsuitable for Flow Cyt,ICC or IHC-P.

ターゲット情報

  • 関連性

    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Apo AIV antibody
      • APOA 4 antibody
      • ApoA IV antibody
      • APOA4 antibody
      • Apolipoprotein A IV antibody
      • Apolipoprotein A4 antibody
      • MGC142154 antibody
      • MGC142156 antibody
      see all

    画像

    • Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200) at 1/1000 dilution + Human plasma at 10 µg

      Predicted band size: 45 kDa

    • Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

      Western blot analysis of APOA4/Apo-AIV in immunoprecipitation pellets from Human plasma (Lane 1) or 1XPBS (negative control) (Lane 2) using ab108200 at 1/10 dilution for IP and at 1/1000 dilution for WB detection.

    • Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

    参考文献 (1)

    ab108200 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108200 は 1 報の論文で使用されています。

    • Cubedo J  et al. ApoL1 levels in high density lipoprotein and cardiovascular event presentation in patients with familial hypercholesterolemia. J Lipid Res 57:1059-73 (2016). PubMed: 27112635

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-APOA4 antibody [EPR2895]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Serum (human plasma)
    Loading amount
    0.5 µg
    Specification
    human plasma
    Gel Running Conditions
    Reduced Denaturing (9%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Hua Jiang

    Verified customer

    投稿 Aug 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com