Anti-Apo-H 抗体 [EPR2898(2)] (ab108348)

Thank you for contacting us. I am sorry to hear you have been experiencing some problems. We have received no other complaints about this product, so in this case it is highly likely that some changes to the protocol will help. Not all antibodies will work under standard conditions, therefore some individual optimisation may be required to help clean up the signal. - Do not dilute the antibody in PBS prior to storage. Simply aliquot into smaller volumes and freeze at -20°C. - Dilute the primary antibody 1:10,000 - 1:50,000. This should reduce the background staining. As this antibody is whole antiserum it isn't unusual to see some background. - Are you using BSA or milk for blocking? BSA can sometimes reduce the background. - How is the primary antibody incubated. I would suggest overnight at 4°C I look forward to your reply.

Thank you for your reply. Please could you provide some further information about how the antibody has been used? I have included some questions below. Order Details Antibody code: Problem Choose: Non-specific band Multiple bands No signal or weak signal High background Lot number Purchase order number or preferably Abcam order number: General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, wrong band size, more bands, no band etc.) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) Amount of protein loaded Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method (ECL, ECLPlus etc.) Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the Western? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place? What steps have you altered? Additional Notes: Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.