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リコンビナントRabMAb

Anti-Apo-D 抗体 [EPR2916] (ab108191)

Submit a review Submit a question References (3)
Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
  • Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
  • Anti-Apo-D antibody [EPR2916] (ab108191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2916] to Apo-D
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Apo-D antibody [EPR2916]
    Apo-D 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2916] to Apo-D

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human plasma cell lysate IHC-P: Human kidney or liver tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108191の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ターゲット情報

  • 機能

    APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

  • 組織特異性

    Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

  • 配列類似性

    Belongs to the calycin superfamily. Lipocalin family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • APO D antibody
      • Apo-D antibody
      • ApoD antibody
      • APOD protein antibody
      • APOD_HUMAN antibody
      • Apolipoprotein D antibody
      • ApolipoproteinD antibody
      see all

    画像

    • Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
      Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
      Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 21 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
      Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
      Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
      Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma cell lysate at 10 µg

      Predicted band size: 21 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)

      Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)

      Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Apo-D antibody [EPR2916] (ab108191)
      Anti-Apo-D antibody [EPR2916] (ab108191)

    参考文献 (3)

    ab108191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108191 は 3 報の論文で使用されています。

    • Wang Y  et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
    • Toyoda Y  et al. Clinical and Molecular Evidence of ABCC11 Protein Expression in Axillary Apocrine Glands of Patients with Axillary Osmidrosis. Int J Mol Sci 18:N/A (2017). PubMed: 28212277
    • Li H  et al. Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice. Neurobiol Aging 36:1820-33 (2015). PubMed: 25784209

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