Application
Flow Cytometry
Sample
Human Cell (Peripheral Blood Mononuclear Cells)
Permeabilization
Yes - 90% Methanol on ice, 20min.
Gating Strategy
FSC v SSC to gate out debris and dying cells followed by CD8+ve selection.
Specification
Peripheral Blood Mononuclear Cells
Preparation
Cell harvesting/tissue preparation method: Cells isolated from whole blood using Dextran/Percoll method.
Sample buffer: 2% FCS
Sample buffer: 2% FCS
Fixation
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 2% FCS in PBS
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H&L), cross absorbed.
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
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投稿 Sep 06 2017