Anti-ATG12 抗体 - N-terminal (ab228494)
Key features and details
- Rabbit polyclonal to ATG12 - N-terminal
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATG12 antibody - N-terminal
ATG12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATG12 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ATG12 (N terminal). An adjuvant has been used to orchestrate/boost the immune response.
Database link: O94817 -
ポジティブ・コントロール
- Flow Cyt (Intra): SH-SY5Y cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7 -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228494の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use 2µg for 106 cells.
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特記事項 |
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Flow Cyt (Intra)
Use 2µg for 106 cells. |
ターゲット情報
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機能
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the ATG12 family. -
ドメイン
Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9140 Human
- Omim: 609608 Human
- SwissProt: O94817 Human
- Unigene: 264482 Human
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別名
- Apg12 antibody
- APG12-like antibody
- APG12L antibody
see all
画像
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Flow cytometric analysis of methanol-fixed, 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling ATG12 with ab228494 at 2 µg per 106 cells (black) compared with a Rabbit IgG Control (white). Donkey anti-Rabbit PE at 1/100 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab228494 は論文での使用が確認できていません。