APC/Cy7® Anti-CD161 抗体 [HP-3G10] (ab270677)

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Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)
  • Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)
  • Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)

Key features and details

  • APC/Cy7® Mouse monoclonal [HP-3G10] to CD161
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
  • Isotype: IgG1

製品の概要

  • 製品名

    APC/Cy7® Anti-CD161 antibody [HP-3G10]
    CD161 一次抗体 製品一覧

  • 製品の詳細

    APC/Cy7® Mouse monoclonal [HP-3G10] to CD161

  • 由来種

    Mouse

  • 標識

    APC/Cy7®. Ex: 650nm, Em: 774nm

  • 特異性

    This antibody recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to CD161. Human NK cells.

  • ポジティブ･コントロール

    • Flow Cyt: Human peripheral whole blood.

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Size exclusion

  • ポリ/モノ

    モノクローナル

  • クローン名

    HP-3G10

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab270677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

ターゲット情報

  • 機能

    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

  • 組織特異性

    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.

  • 配列類似性

    Contains 1 C-type lectin domain.

  • 翻訳後修飾

    N-glycosylated. Contains sialic acid residues.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    画像

    • Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)
      Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)

      Flow cytometry analysis of human peripheral whole blood labeling CD161 positive lymphocytes using ab270677 (Red). Surface staining.

      Control: Human neutrophil granulocytes (Black).

    • Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)
      Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)

      Flow cytometry analysis of human lymphocytes labeling CD161 using ab270677 (bottom cluster). Surface staining.

      Control is an anti-CD4 (MEM-241) FITC antibody (top cluster) at 20 μl reagent in 100 μl of peripheral whole blood.

    • Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)
      Flow Cytometry - APC/Cy7® Anti-CD161 antibody [HP-3G10] (ab270677)

      Flow cytometry analysis of human peripheral whole blood labeling CD161 using ab270677. Surface staining.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270677 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270677 は論文での使用が確認できていません。

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