Anti-Apc4 抗体 (ab72149)
Key features and details
- Rabbit polyclonal to Apc4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Apc4 antibody
Apc4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Apc4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region between residues 758 and 808 of human Apc4 (NP_037499.2).
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ポジティブ・コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72149の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the APC4 family. - Information by UniProt
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参照データベース
- Entrez Gene: 29945 Human
- Entrez Gene: 52206 Mouse
- Entrez Gene: 305420 Rat
- Omim: 606947 Human
- SwissProt: Q9UJX5 Human
- SwissProt: Q91W96 Mouse
- Unigene: 152173 Human
- Unigene: 272568 Mouse
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別名
- ANAPC 4 antibody
- anapc4 antibody
- Anaphase Promoting Complex Subunit 4 antibody
see all
画像
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All lanes : Anti-Apc4 antibody (ab72149) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab72149 は 8 報の論文で使用されています。
- Wang Y & Poon RYC MARCH5 regulates mitotic apoptosis through MCL1-dependent and independent mechanisms. Cell Death Differ 30:753-765 (2023). PubMed: 36329234
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Lau HW et al. Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells. Cell Rep 37:109808 (2021). PubMed: 34644583
- Alfieri C et al. A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase. EMBO Rep 21:e49831 (2020). PubMed: 32307883
- Zhang S et al. Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C. Nat Commun 10:3863 (2019). PubMed: 31455778
- Masuda K et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS One 10:e0118662 (2015). PubMed: 25723520
- Ma HT & Poon RY Orderly Inactivation of the Key Checkpoint Protein Mitotic Arrest Deficient 2 (MAD2) during Mitotic Progression. J Biol Chem 286:13052-9 (2011). WB ; Human . PubMed: 21335556
- Mo M et al. Cell cycle deregulation by a poxvirus partial mimic of anaphase-promoting complex subunit 11. Proc Natl Acad Sci U S A 106:19527-32 (2009). WB ; Human . PubMed: 19887645