Anti-Apc1 (phospho S355) 抗体 (ab10923)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S355)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Apc1 (phospho S355) antibody
Apc1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Apc1 (phospho S355) -
由来種
Rabbit -
特異性
Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human Apc1 aa 350-450 (phospho S355) conjugated to keyhole limpet haemocyanin.
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特記事項
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
一次抗体 備考
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10923の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
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| 特記事項 |
|---|
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WB
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
ターゲット情報
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機能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the APC1 family.
Contains 4 PC repeats. -
翻訳後修飾
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 535565 Cow
- Entrez Gene: 475750 Dog
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: E1BAB6 Cow
- SwissProt: E2RLM2 Dog
- SwissProt: Q9H1A4 Human
see all -
別名
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
画像
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Western blot - Anti-Apc1 (phospho S355) antibody (ab10923)All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDa
Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab10923 は 1 報の論文で使用されています。
- Zhang Y et al. miR-29a suppresses IL-13-induced cell invasion by inhibiting YY1 in the AKT pathway in lung adenocarcinoma A549 cells. Oncol Rep 39:2613-2623 (2018). PubMed: 29620222