APC Anti-Thymidylate Synthase 抗体 [EPR4545] (ab225091)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR4545] to Thymidylate Synthase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
製品の概要
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製品名
APC Anti-Thymidylate Synthase antibody [EPR4545]
Thymidylate Synthase 一次抗体 製品一覧 -
製品の詳細
APC Rabbit monoclonal [EPR4545] to Thymidylate Synthase -
由来種
Rabbit -
標識
APC. Ex: 645nm, Em: 660nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (intra): HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4545 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
- Alexa Fluor® 488 Anti-Thymidylate Synthase antibody [EPR4545] (ab197898)
- Alexa Fluor® 647 Anti-Thymidylate Synthase antibody [EPR4545] (ab197899)
- PE Anti-Thymidylate Synthase antibody [EPR4545] (ab225092)
- Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (ab232021)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab225091の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
特記事項 |
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Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
ターゲット情報
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機能
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
パスウェイ
Pyrimidine metabolism; dTTP biosynthesis. -
配列類似性
Belongs to the thymidylate synthase family. -
細胞内局在
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7298 Human
- Entrez Gene: 22171 Mouse
- Entrez Gene: 29261 Rat
- Omim: 188350 Human
- SwissProt: P04818 Human
- SwissProt: P07607 Mouse
- SwissProt: P45352 Rat
- Unigene: 369762 Human
see all -
別名
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
画像
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Overlay histogram showing HeLa cells stained with ab225091 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225091, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab225091 は論文での使用が確認できていません。