APC Anti-Sialoadhesin/CD169 抗体 [7-239] (ab272341)
![Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)](https://www.abcam.co.jp/ps/products/272/ab272341/Images/ab272341-387920-Mouse-monoclonal-7239-Sialoadhesin-CD169-APC-flow-cytometry-tnf-alpha-gamma-stimulated-pbmc-human1.jpg "Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)")
Key features and details
- APC Mouse monoclonal [7-239] to Sialoadhesin/CD169
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
製品の概要
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製品名
APC Anti-Sialoadhesin/CD169 antibody [7-239]
Sialoadhesin/CD169 一次抗体 製品一覧 -
製品の詳細
APC Mouse monoclonal [7-239] to Sialoadhesin/CD169 -
由来種
Mouse -
標識
APC. Ex: 645nm, Em: 660nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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免疫原
Tissue, cells or virus. Human rhinovirus 14-infected monocyte-derived dendritic cells.
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エピトープ
Extracellular -
ポジティブ･コントロール
- Flow Cyt: TNF-alpha and INF-gamma stimulated human peripheral blood mononuclear cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Size exclusion -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
7-239 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
ターゲット情報
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機能
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. -
組織特異性
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6614 Human
- Omim: 600751 Human
- SwissProt: Q9BZZ2 Human
- Unigene: 31869 Human
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別名
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
see all
画像
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Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)
Flow cytometry surface staining pattern of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using ab272341 at 10 μl reagent per milion cells in 100 μl of cell suspension.
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![Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)](https://www.abcam.co.jp/ps/products/272/ab272341/Images/ab272341-387919-Mouse-monoclonal-7239-Sialoadhesin-CD169-APC-flow-cytometry-tnf-alpha-gamma-stimulated-pbmc-human2.jpg)
Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)Flow cytometry multicolor surface staining of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using ab272341 at 10 μl reagent per milion cells in 100 μl of cell suspension and anti-human CD11c (BU15) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
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![Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)](https://www.abcam.co.jp/ps/products/272/ab272341/Images/ab272341-387918-Mouse-monoclonal-7239-Sialoadhesin-CD169-APC-flow-cytometry-tnf-alpha-gamma-stimulated-pbmc-human3.jpg)
Flow Cytometry - APC Anti-Sialoadhesin/CD169 antibody [7-239] (ab272341)Separation of human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human TNF-α and INF-γ stimulated peripheral blood mononuclear cells stained using ab272341 at 10 μl reagent per milion cells in 100 μl of cell suspension.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272341 は論文での使用が確認できていません。