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APC Anti-CD19 抗体 [LT19] (ab18224)

Submit a review Q&A (1)References (5)

Key features and details

  • APC Mouse monoclonal [LT19] to CD19
  • Suitable for: IP, Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG1

製品の概要

  • 製品名

    APC Anti-CD19 antibody [LT19]
    CD19 一次抗体 製品一覧

  • 製品の詳細

    APC Mouse monoclonal [LT19] to CD19

  • 由来種

    Mouse

  • 標識

    APC. Ex: 645nm, Em: 660nm

  • アプリケーション

    適用あり: IP, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD19. B lymphoblast cell line Daudi

  • ポジティブ･コントロール

    • Human blood cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18224の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Flow Cyt
特記事項
追加情報
Flow Cyt: Use 20µl for 100µl human blood cells.
IP: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能

    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

  • 関連疾患

    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antibody deficiency due to defect in CD19 antibody
      • Antibody deficiency due to defect in CD19, included antibody
      • AW495831 antibody
      • B lymphocyte antigen CD19 antibody
      • B lymphocyte surface antigen B4 antibody
      • B-lymphocyte antigen CD19 antibody
      • B-lymphocyte surface antigen B4 antibody
      • B4 antibody
      • CD19 antibody
      • CD19 antigen antibody
      • CD19 molecule antibody
      • Cd19 protein antibody
      • CD19_HUMAN antibody
      • CVID3 antibody
      • Differentiation antigen CD19 antibody
      • Leu 12 antibody
      • Leu-12 antibody
      • Leu12 antibody
      • MGC109570 antibody
      • MGC12802 antibody
      • T-cell surface antigen Leu-12 antibody
      see all

    参考文献 (5)

    ab18224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18224 は 5 報の論文で使用されています。

    • Li GQ  et al. MicroRNA-21 from bone marrow mesenchymal stem cell-derived extracellular vesicles targets TET1 to suppress KLF4 and alleviate rheumatoid arthritis. Ther Adv Chronic Dis 12:20406223211007369 (2021). PubMed: 33995992
    • Ding F  et al. microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer. Stem Cell Res Ther 11:455 (2020). PubMed: 33109266
    • Jiang Y  et al. Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice. Front Med (Lausanne) 7:577578 (2020). PubMed: 33425934
    • Liu L  et al. Pfit is a structurally novel Crohn's disease-associated superantigen. PLoS Pathog 9:e1003837 (2013). PubMed: 24385909
    • Trepel M  et al. Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma. PLoS One 7:e31998 (2012). Flow Cyt ; Human . PubMed: 22384124

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Dear all,
    or a flow cytometry approach I need an anti human CD19 antibody.
    I have found the ab18224, but the problem is you do not have the corresponding isotype control for this? Is this correct?

    Read More
    Answer



    Ab18224 is a suitable alternative to target CD19 with Flow Cytometry. I’ve conducted a search for an isotype control and found the following product that matches the requirements needed: https://www.abcam.com/mouse-igg1-1f8-allophycocyanin-isotype-control-ab91358.html.

    Read More

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