Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cardiac fibroblasts)
Gel Running Conditions
Reduced Denaturing (8.5%)
Loading amount
20 µg
Specification
primary cardiac fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 140 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 11 2019