Anti-APAF1 抗体 (ab2001)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-APAF1 antibody
APAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to APAF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human APAF1 aa 12-28 (N terminal).
Sequence:HREALEKDIKTSYIMDHC
(Peptide available asab7873) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 1 µg/ml.
|
WB | (3) |
1/1000 - 1/2000. Detects a band of approximately 141 kDa.
|
特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/2000. Detects a band of approximately 141 kDa. |
ターゲット情報
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機能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
組織特異性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
配列類似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
ドメイン
The CARD domain mediates interaction with APIP. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 317 Human
- Omim: 602233 Human
- SwissProt: O14727 Human
- Unigene: 728891 Human
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別名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab2001 は 29 報の論文で使用されています。
- Luo M et al. H Long Terminal Repeat-Associating 2 (HHLA2) is a Biomarker of Advanced Stage Hepatocellular Carcinoma and Promotes Tumor Cell Development In Vitro. Med Sci Monit 27:e930215 (2021). PubMed: 33990536
- Guo Z et al. MiR-502 Suppresses TNF-a-Induced Nucleus Pulposus Cell Apoptosis by Targeting TARF2. Biomed Res Int 2021:5558369 (2021). PubMed: 33869626
- Cavalcante RS et al. STAT3/NF-?B signalling disruption in M2 tumour-associated macrophages is a major target of PLGA nanocarriers/PD-L1 antibody immunomodulatory therapy in breast cancer. Br J Pharmacol 178:2284-2304 (2021). PubMed: 33434950
- Yue M et al. NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation. RNA Biol 18:1981-1995 (2021). PubMed: 33530829
- Wang C et al. Combinative treatment of Curdione and docetaxel triggers reactive oxygen species (ROS)-mediated intrinsic apoptosis of triple-negative breast cancer cells. Bioengineered 12:10037-10048 (2021). PubMed: 34666596