Anti-APAF1 抗体 (ab2000)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-APAF1 antibody
APAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to APAF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).
Sequence:TFYTNGTNLKKIHVSPDFKT
(Peptide available asab7872) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 20 µg/ml.
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 130 kDa.
|
特記事項 |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
1/500 - 1/2000. Predicted molecular weight: 130 kDa. |
ターゲット情報
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機能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
組織特異性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
配列類似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
ドメイン
The CARD domain mediates interaction with APIP. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 317 Human
- Omim: 602233 Human
- SwissProt: O14727 Human
- Unigene: 728891 Human
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別名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
画像
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All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml
Lane 1 : Human heart tissue lysate with absence of blocking peptide
Lane 2 : Human heart tissue lysate with presence of blocking peptide
Predicted band size: 130 kDa
Observed band size: 130 kDa -
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab2000 は 10 報の論文で使用されています。
- Gui C et al. Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF-7 cell line. Int J Oncol 58:409-418 (2021). PubMed: 33469684
- Zhang X et al. The effects of umbilical cord-derived macrophage exosomes loaded with cisplatin on the growth and drug resistance of ovarian cancer cells. Drug Dev Ind Pharm N/A:1-13 (2020). PubMed: 32482115
- Shao S et al. Toosendanin induces apoptosis of MKN-45 human gastric cancer cells partly through miR-23a-3p-mediated downregulation of BCL2. Mol Med Rep 22:1793-1802 (2020). PubMed: 32582989
- Hu X et al. The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells. Front Oncol 10:558414 (2020). PubMed: 33178586
- Pan Z et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
- Wang SQ et al. Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells. Mol Ther Nucleic Acids 16:105-117 (2019). PubMed: 30861413
- Liu Y et al. Breviscapine ameliorates CCl4-induced liver injury in mice through inhibiting inflammatory apoptotic response and ROS generation. Int J Mol Med 42:755-768 (2018). PubMed: 29717768
- Gulic T et al. Granulysin-mediated apoptosis of trophoblasts in blighted ovum and missed abortion. Am J Reprod Immunol 80:e12978 (2018). PubMed: 29774968
- Hathaway QA et al. Maternal-engineered nanomaterial exposure disrupts progeny cardiac function and bioenergetics. Am J Physiol Heart Circ Physiol 312:H446-H458 (2017). PubMed: 28011589
- Nichols CE et al. Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Am J Physiol Heart Circ Physiol 309:H2017-30 (2015). PubMed: 26497962