Application
Western blot
Sample
Human Cell lysate - nuclear (Hela)
Loading amount
30 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Rotiblock
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti-mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 48 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jul 30 2008