Anti-AP2 alpha + beta 抗体 [A6/2/2] (ab61)

Reviews (1)Q&A (6)References (2)
Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)

    Key features and details

    • Mouse monoclonal [A6/2/2] to AP2 alpha + beta
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-AP2 alpha + beta antibody [A6/2/2]

    • 製品の詳細

      Mouse monoclonal [A6/2/2] to AP2 alpha + beta

    • 由来種

      Mouse

    • 特異性

      Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.

    • アプリケーション

      適用あり: ICC/IFmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human AP2 alpha + beta (C terminal) conjugated to keyhole limpet haemocyanin.
      Database link: P05549

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Raji cells or breast carcinoma

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      A6/2/2

    • ミエローマ

      NS0

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab61の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    Use a concentration of 1 µg/ml.
    特記事項
    ICC/IF
    Use a concentration of 1 µg/ml.

    ターゲット情報

    • 関連性

      The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia

    • 細胞内局在

      Nuclear

    • 参照データベース

      • 別名

        • Activating enhancer binding protein 2 alpha antibody
        • Activating enhancer binding protein 2 beta antibody
        • Activating enhancer-binding protein 2-alpha antibody
        • Activator protein 2 antibody
        • AP 2 antibody
        • AP 2alpha antibody
        • AP 2B antibody
        • AP-2 antibody
        • AP-2 transcription factor antibody
        • AP2 B antibody
        • AP2-alpha antibody
        • AP2-beta antibody
        • AP2TF antibody
        • BOFS antibody
        • TFAP2 antibody
        • TFAP2A antibody
        • TFAP2B antibody
        • Transcription factor AP2 alpha antibody
        • Transcription factor AP2 beta antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)
        Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)
        ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      参考文献 (2)

      ab61 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab61 は 2 報の論文で使用されています。

      • Chen J  et al. Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer. Genome Biol 21:152 (2020). PubMed: 32580738
      • Gee JM  et al. Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer. J Pathol 189:514-20 (1999). PubMed: 10629551

      レビューと Q&A

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      1-7 of 7 Abreviews or Q&A

      Western blot abreview for Anti-AP2 alpha + beta antibody [A6/2/2]

       Good
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - nuclear (Hela)
      Loading amount
      30 µg
      Specification
      Hela
      Gel Running Conditions
      Reduced Denaturing
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      Abcam user community

      Verified customer

      投稿 Jul 30 2008

      Question

      Do you have a recommendation for a positive control tissue for this antibody?

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      Answer

      Thank you for your enquiry. Raji cells or breast carcinomas should be a suitable positive control. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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      Question

      Do you know the exact amino acid sequence of the immunogen?

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      Answer

      The antibody A6/2/2 was raised against the peptide PNSHTDNNAKSSDKEEKHRK which is the C-terminus of AP-2alpha. As the antibody also recognises AP 2beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta. The antibody is described in: Gee, J.M.W., Robertson, J.F.R., Ellis,I.O., Nicholson,R.I. and Hurst,H.C.(1999). Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in breast cancer. J. Pathol. 189, 514-520

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      Question

      Do you know the recommended dilutions for western analysis?

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      Answer

      We always recommend that conditions for WB should be optimised by the end user. We suggest starting with a 1:1000 dilution.

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      Question

      Hi, Is this AP-2, the adaptor protein (AP-2) which is important in vesicle formation? Cheers Chris Partridge School of Biomedical Sciences University of Leeds UK

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      Answer

      This antibody recognises the transcription factor AP-2 and thus not the adaptor protein (AP-2).

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      Question

      Does this work in gel shift (EMSA) assays??

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      Answer

      We don't have any information on this antibody being used in EMSA, but as the immunogen was a C-terminal peptide chances are quite high it will work.

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      Question

      My name is Estibaliz Lopez and I am a Ph.D. student at Uppsala University in Sweden. I would like to know if this AP-2 antibody will be useful for whole mount immunohistochemistry. We fix our mouse embryos in 4% paraformaldehyde overnight. Have you try this before?. If so, does it work?. Thanks for your attention. Estibaliz.

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      Answer

      This antibody works in immunohistochemistry with paraffin embedded sections (fixative formal saline with calcium, 10 min microwave treatment required). However it has not been tested for whole mount IHC as you describe.

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