Anti-AP2 alpha + beta 抗体 [A6/2/2] (ab61)

Reviews (1)Q&A (6)References (1)

製品の概要

  • 製品名

    Anti-AP2 alpha + beta antibody [A6/2/2]

  • 製品の詳細

    Mouse monoclonal [A6/2/2] to AP2 alpha + beta

  • 由来種

    Mouse

  • 特異性

    Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.

  • アプリケーション

    適用あり: Flow Cyt, WB, ELISA, IHC-P, RIA, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    C-terminal peptide of AP-2 alpha (PNSHTDNNAKSSDKEEKHRK).

  • ポジティブ･コントロール

    • Raji cells or breast carcinoma

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    A6/2/2

  • ミエローマ

    NS0

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • Activating enhancer binding protein 2 alpha antibody
      • Activating enhancer binding protein 2 beta antibody
      • Activator protein 2 antibody
      • AP 2 antibody
      • AP 2alpha antibody
      • AP 2B antibody
      • AP-2 transcription factor antibody
      • AP2 B antibody
      • AP2-alpha antibody
      • AP2-beta antibody
      • AP2TF antibody
      • BOFS antibody
      • TFAP2 antibody
      • TFAP2A antibody
      • TFAP2B antibody
      • Transcription factor AP2 alpha antibody
      • Transcription factor AP2 beta antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)
      Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)
      ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    This product has been referenced in:

    • Gee JM  et al. Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer. J Pathol 189:514-20 (1999). Read more (PubMed: 10629551) »
    See 1 Publication for this product

    ab61 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-AP2 alpha + beta antibody [A6/2/2]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela)
    Loading amount
    30 µg
    Specification
    Hela
    Gel Running Conditions
    Reduced Denaturing
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 30 2008

    Question

    Do you have a recommendation for a positive control tissue for this antibody?

    Read More
    Answer

    Thank you for your enquiry. Raji cells or breast carcinomas should be a suitable positive control. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Do you know the exact amino acid sequence of the immunogen?

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    Answer

    The antibody A6/2/2 was raised against the peptide PNSHTDNNAKSSDKEEKHRK which is the C-terminus of AP-2alpha. As the antibody also recognises AP 2beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta. The antibody is described in: Gee, J.M.W., Robertson, J.F.R., Ellis,I.O., Nicholson,R.I. and Hurst,H.C.(1999). Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in breast cancer. J. Pathol. 189, 514-520

    Read More

    Question

    Do you know the recommended dilutions for western analysis?

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    Answer

    We always recommend that conditions for WB should be optimised by the end user. We suggest starting with a 1:1000 dilution.

    Read More

    Question

    Hi, Is this AP-2, the adaptor protein (AP-2) which is important in vesicle formation? Cheers Chris Partridge School of Biomedical Sciences University of Leeds UK

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    Answer

    This antibody recognises the transcription factor AP-2 and thus not the adaptor protein (AP-2).

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    Question

    Does this work in gel shift (EMSA) assays??

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    Answer

    We don't have any information on this antibody being used in EMSA, but as the immunogen was a C-terminal peptide chances are quite high it will work.

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    Question

    My name is Estibaliz Lopez and I am a Ph.D. student at Uppsala University in Sweden. I would like to know if this AP-2 antibody will be useful for whole mount immunohistochemistry. We fix our mouse embryos in 4% paraformaldehyde overnight. Have you try this before?. If so, does it work?. Thanks for your attention. Estibaliz.

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    Answer

    This antibody works in immunohistochemistry with paraffin embedded sections (fixative formal saline with calcium, 10 min microwave treatment required). However it has not been tested for whole mount IHC as you describe.

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