Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5371] to Antithrombin III/ATIII - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
製品名Anti-Antithrombin III/ATIII antibody [EPR5371] - BSA and Azide free
Antithrombin III/ATIII 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5371] to Antithrombin III/ATIII - BSA and Azide free
アプリケーション適用あり: ICC/IF, WBmore details
適用なし: IHC-P or IP
非交差種: Mouse, Rat
Synthetic peptide within Human Antithrombin III/ATIII aa 1-100. The exact sequence is proprietary.
Ab248012 is the carrier-free version of ab124808. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248012 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
機能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
組織特異性Found in plasma.
関連疾患Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
配列類似性Belongs to the serpin family.
翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
細胞内局在Secreted > extracellular space.
- Information by UniProt
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
All lanes : Anti-Antithrombin III/ATIII antibody [EPR5371] (ab124808) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This data was developed using ab124808, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.