Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)
製品の概要
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製品名
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
PROTOCOL:
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
製品の特性
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製品の状態
Liquid -
保存方法
Store at room temperature. -
バッファー
pH: 8
Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA
Buffer 100x Concentrated with detergent. -
Concentration information loading...
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent dilution.
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特記事項 |
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IHC-P
Use at an assay dependent dilution. |
画像
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Prolonged survival of Lgr5/Apc and MYC-ON mice depends on the T cells.g Immunohistochemical staining of GFP in the indicated genotypes (n = 5 mice per group).
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Ophiocordyceps formosana upregulated adiponectin expressions in plasma and adipose tissuesb) Immunohistochemical staining for adiponectin in adipose tissues.
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ab93680 Antigen Retrieval Buffer 100X EDTA Buffer, pH 8
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab93680 は 4 報の論文で使用されています。
- Wang Y et al. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. Elife 9:N/A (2020). PubMed: 32479261
- Wang G et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun 11:220 (2020). PubMed: 31924786
- Huang CW et al. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model. BMC Complement Altern Med 16:310 (2016). IHC-P . PubMed: 27553852
- Zhu Y et al. Elevated expression of AKT2 correlates with disease severity and poor prognosis in human osteosarcoma. Mol Med Rep 10:737-42 (2014). PubMed: 24919955