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Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)

Submit a review Q&A (3)References (2)

製品の概要

  • 製品名

    Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent
    Annexin V/ANXA5 ihc 試薬 製品一覧

  • アプリケーション

    適用あり: FM, Flow Cytmore details

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called 1013 Annexin V-Cy5 Reagent.

    Annexin V/ANXA5-Cy5 Apoptosis Detection Reagent ab14147 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-Cy5 reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy5 reagent is also available as part of Annexin V/ANXA5-Cy5 apoptosis kit ab14150.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14147の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
特記事項
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.

参考文献 (2)

ab14147 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14147 は 2 報の論文で使用されています。

  • Guo X  et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nat Med 24:978-985 (2018). PubMed: 29942094
  • Wu KW  et al. Neurovascular Interaction Promotes the Morphological and Functional Maturation of Cortical Neurons. Front Cell Neurosci 11:290 (2017). PubMed: 28966577

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hello, I amm testing Cy5-Annexin-V reagents. Can you please tell me whether the annexin used in the preparation of this product is a recombinant protein? Some vendors provide material isolated from human placenta and some use E.Coli generated material. Many thanks,

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Answer

Thank you for contacting us.

The source is E.Coli and it is a recombinant Annexin V.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi,   I recently purchased Annexin V-Cy5 ab14147 from you and had a question that wasn’t covered in the pack literature that came with it.   What is the source of Annexin (i.e. Human, recombinant etc.)? Regards,

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Answer

Thank you for contacting us. The source is E.Coli and it is a recombinant Annexin V. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

Good day     Please provide us with a Certificate of Analysis and a Certificate of Origin for :   AB14147 Lot # GR88608-1

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Answer

Thank you for your enquiry.Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within six month of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

I am happy to confirm that I attached the CoC to this email.

As for the "Certificate of origin", I regret to inform you that we do not provide it, because in addition to our in-house products, there are some products which are supplied to us from outside collaborators, just as in the case of Annexin V-Cy5 Apoptosis Detection Reagentab14147. I can however confirm that we do source is from a collaborating laboratory in the USA.

Please do not hesitate to contact me again if you have further questions.

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