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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Annexin V/ANXA5 抗体 [EPR3980] (ab108194)

Submit a review Q&A (1)References (4)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
  • Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
  • Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3980] to Annexin V/ANXA5
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Annexin V/ANXA5 antibody [EPR3980]
    Annexin V/ANXA5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3980] to Annexin V/ANXA5

  • 由来種

    Rabbit

  • 特異性

    The immunogen used for this product shares 80% homology with ANXA2 and 73% homology with ANXA4. Cross-reactivity with this protein has not been confirmed experimentally.

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (Intra): HeLa cells WB: HeLa, HAP1, and Jurkat cell lysates, and Mouse brain, Mouse heart, Rat Brain, and Rat heart tissue lysates ICC: JAR (Human placenta choriocarcinoma epithelial cell) cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108194の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 36 kDa.
ICC/IF
1/50.

For unpurified use at 1/500 - 1/1000.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/1000.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 36 kDa.
ICC/IF
1/50.

For unpurified use at 1/500 - 1/1000.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/1000.

ターゲット情報

  • 機能

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

  • 関連疾患

    Pregnancy loss, recurrent, 3

  • 配列類似性

    Belongs to the annexin family.
    Contains 4 annexin repeats.

  • ドメイン

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.

  • 翻訳後修飾

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Anchorin CII antibody
    • Annexin 5 antibody
    • Annexin A5 antibody
    • Annexin V antibody
    • Annexin-5 antibody
    • Annexin5 antibody
    • AnnexinA5 antibody
    • AnnexinV antibody
    • ANX 5 antibody
    • ANX A5 antibody
    • ANX5 antibody
    • ANXA5 antibody
    • ANXA5_HUMAN antibody
    • Calphobindin I antibody
    • CBP-I antibody
    • Endonexin II antibody
    • ENX 2 antibody
    • ENX2 antibody
    • Lipocortin V antibody
    • PAP I antibody
    • PAP-I antibody
    • Placental anticoagulant protein 4 antibody
    • Placental anticoagulant protein I antibody
    • PLACENTAL PROTEIN 4 antibody
    • PP 4 antibody
    • Pp4 antibody
    • RPRGL3 antibody
    • Thromboplastin inhibitor antibody
    • VAC-alpha antibody
    • Vascular anticoagulant-alpha antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunocytochemistry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Annexin V/ANXA5 with purified ab108194 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab108194 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108194 was shown to recognize Annexin V in wild-type cells as signal was lost at the expected MW in Annexin V knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab108194 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Annexin V/ANXA5 with purified ab108194 at 1/30 dilution (10 µg/ml) (red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    All lanes : Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : Mouse brain
    Lane 4 : Mouse heart
    Lane 5 : Rat brain
    Lane 6 : Rat heart

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

参考文献 (4)

ab108194 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108194 は 4 報の論文で使用されています。

  • Pecan P  et al. Calcium Ionophore-Induced Extracellular Vesicles Mediate Cytoprotection against Simulated Ischemia/Reperfusion Injury in Cardiomyocyte-Derived Cell Lines by Inducing Heme Oxygenase 1. Int J Mol Sci 21:N/A (2020). PubMed: 33081396
  • Lin Q  et al. Exosome-like nanoplatform modified with targeting ligand improves anti-cancer and anti-inflammation effects of imperialine. J Control Release 311-312:104-116 (2019). PubMed: 31484040
  • Middleton RC  et al. Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes. J Extracell Vesicles 7:1456888 (2018). PubMed: 29696078
  • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). ICC/IF ; Human . PubMed: 22347520

レビューと Q&A

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レビューを送る 質問を送る

Question

Currently, I am using the abcam Annexin V antibody (ab54775) and I would like to know whether this antibody is capable to block the Annexin V (e.g. on cell surfaces). If yes, I would be interested to get some references if possibleI. If not, could you offer me a product to block Annexin V (either a blocking AV antibody or maybe a soluble phosphatidylserine).

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Answer

Thank you for contacting us and for your patience.

I have contacted the originator of anti-Annexin V antibody ab54775 and our other anti-Annexin V antibodies:

Unfortunately, it is not known whether ab54775 is capable of blocking Annexin V as this is not normally tested. Generally, it is testedif the antibody binding can be blocked, for example by using the immunogen - in this caserecombinant protein could be used to block antibody. However, we have no experience in using recombinant protein or other materials to block this antibody.
As the expression system of the recombinant protein was cell-free protein synthesis system (in vitro wheat germ protein expression system). Glycosylation and other natural eukaryotic modifications of synthesized protein do not take place in this cell-free system. Also, we are not sure whether recombinant protein will be folded correctly.

In general, we do not know whether any of our antibodies could block the Annexin V binding. The antibodies are specific for different domains of human Annexin V (for example ab108194 amino acids 296 - 320 which is in the fourth Annexin domain, http://www.uniprot.org/uniprot/P08758). If the binding to these domainsis sufficient to block Annexin V though, has not been determined yet.

Also, we currently do not have other products such as soluble phosphatidylserine in our catalogue which might be suitable for your experiments.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We would encourage you to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research interests.

Should you decide to go ahead and test one of ourAnnexin V antibodies in blocking experiments, we encourage you to submit an Abreview of your results. We appreciate all customer feedback, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews

I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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