Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Asynchronous HeLa treated with bleomycin (DNA double-strand break inducer) were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected membrane-associated staining pattern. Methanol-fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab108321 and it seems that this antibody does not recognize Annexin V under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Sep 04 2012