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    annexin-vanxa5-antibody-ab14196.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Anti-Annexin V/ANXA5 抗体 (ab14196)

  • Datasheet
  • SDS
Reviews (19)Q&A (5)References (76)

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Western blot - Anti-Annexin V/ANXA5 antibody (ab14196)

    Key features and details

    • Rabbit polyclonal to Annexin V/ANXA5
    • Suitable for: IHC-P, IHC-Fr, IHC-FoFr, ICC/IF, WB, Flow Cyt
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Annexin V/ANXA5 antibody
      Annexin V/ANXA5 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Annexin V/ANXA5
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-P, IHC-Fr, IHC-FoFr, ICC/IF, WB, Flow Cytmore details
    • 種交差性

      交差種: Mouse, Rat, Human
    • 免疫原

      Recombinant full length protein corresponding to Human Annexin V/ANXA5.
      Database link: P08758
      (Peptide available as ab52111)

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called 3357 Annexin V Antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • バッファー

      Preservative: 0.03% Proclin 300
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Cell Biology
      • Apoptosis
      • Annexin V Reagents
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Detection kits
      • Annexin V
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Detection Kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Annexin V
      • Annexin V Reagents

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Annexin V/ANXA5 peptide (ab52111)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Annexin V/ANXA5 protein (His tag) (ab157342)
    • Related Products

      • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
      • Propidium Iodide (ab14083)
      • 10X Annexin V/ANXA5 Binding Buffer (ab14084)
      • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
      • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
      • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
      • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
      • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
      • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
      • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
      • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
      • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
      • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
      • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
      • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab14196の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (3)
    Use at an assay dependent concentration.
    IHC-Fr (2)
    Use at an assay dependent concentration.
    IHC-FoFr (1)
    Use at an assay dependent concentration.
    ICC/IF (3)
    Use at an assay dependent concentration. PubMed: 20086251
    WB (8)
    Use a concentration of 1 - 4 µg/ml. Can be blocked with Annexin V/ANXA5 peptide (ab52111).
    Flow Cyt (1)
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

    特記事項
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-FoFr
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 20086251
    WB
    Use a concentration of 1 - 4 µg/ml. Can be blocked with Annexin V/ANXA5 peptide (ab52111).
    Flow Cyt
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    • 関連疾患

      Pregnancy loss, recurrent, 3
    • 配列類似性

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • ドメイン

      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • 翻訳後修飾

      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Target information above from: UniProt accession P08758 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 308 Human
      • Entrez Gene: 11747 Mouse
      • Entrez Gene: 25673 Rat
      • Omim: 131230 Human
      • SwissProt: P08758 Human
      • SwissProt: P48036 Mouse
      • SwissProt: P14668 Rat
      • Unigene: 480653 Human
      • Unigene: 1620 Mouse
      • Unigene: 3318 Rat
      see all
    • 別名

      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • Annexin5 antibody
      • AnnexinA5 antibody
      • AnnexinV antibody
      • ANX 5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX 2 antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • PP 4 antibody
      • Pp4 antibody
      • RPRGL3 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    画像

    • Western blot - Anti-Annexin V/ANXA5 antibody (ab14196)
      Western blot - Anti-Annexin V/ANXA5 antibody (ab14196)
      Anti-Annexin V/ANXA5 antibody (ab14196) + HeLa cell lysate

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (76)

    ab14196 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14196 は 76 報の論文で使用されています。

    • Marsilio S  et al. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma. J Vet Intern Med 35:179-189 (2021). PubMed: 33471936
    • Feige P  et al. Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers. Skelet Muscle 11:1 (2021). PubMed: 33397479
    • Haeckel A  et al. Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications. Apoptosis 26:534-547 (2021). PubMed: 34405304
    • Kumar M  et al. Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PARKIN-Deficient Human Dopamine Neurons. Stem Cell Reports 15:629-645 (2020). PubMed: 32795422
    • Tian M  et al. Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation. Small 16:e1904064 (2020). PubMed: 32067382
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 24 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Annexin V/ANXA5 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Jurkat cells)
    Permeabilization
    No
    Gating Strategy
    no gate
    Specification
    Jurkat cells
    Preparation
    Cell harvesting/tissue preparation method: cell culture
    Sample buffer: Binding buffer
    Fixation
    none
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Annexin V antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Jurkat cells)
    Permeabilization
    No
    Specification
    Jurkat cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin V/ANXA5 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrat buffer ph6,0
    Permeabilization
    Yes - 0,2% Triton/PBS
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Annexin V antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Jaculus jaculus Tissue sections (Skin)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mai Tran

    Verified customer

    投稿 Nov 04 2016

    Western blot abreview for Anti-Annexin V/ANXA5 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huamn)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    alpha synuclein
    Specification
    huamn
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Ms. Sehee Oh

    Verified customer

    投稿 Jul 13 2016

    Western blot abreview for Anti-Annexin V antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HBMEC (Human Brain Microvascular Endothelial Cell))
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    HBMEC (Human Brain Microvascular Endothelial Cell)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 31 2015

    Western blot abreview for Anti-Annexin V/ANXA5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Pericytes)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Pericytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 28 2015

    Western blot abreview for Anti-Annexin V antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (bEnd.3)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    bEnd.3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 07 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Annexin V/ANXA5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Embryonic 15 day brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    Embryonic 15 day brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 23 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Annexin V antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Mouse Tissue sections (lumbar spinal cord, 25 micron)
    Specification
    lumbar spinal cord, 25 micron
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 04 2014

    1-10 of 24 Abreviews or Q&A

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