Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
Key features and details
- Assay type: Direct
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 10 min
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Annexin V-Cy5 Apoptosis Staining / Detection Kit
Annexin V/ANXA5 キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Direct -
全工程の試験時間
0h 10m -
製品の概要
Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.
Fluorescence can be detected at Ex/Em = 649/670 nm.
Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent ab14147.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K103 Annexin V-Cy5 Apoptosis Kit. K103-100 is the same size as the 100 test size of ab14150.
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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試験プラットフォーム
Flow cytometer, Fluorescence microscope
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 100 tests Annexin V Cy5 1 x 500µl Binding Buffer 1 x 50ml -
研究分野
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機能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
関連疾患
Pregnancy loss, recurrent, 3 -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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別名
- Anchorin CII
- Annexin 5
- Annexin A5
see all
関連製品
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画像
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Impact of different Rep constructs on DNA damage response and apoptosis - ab14150Image from Seyffert M et al., PLoS One 12(1), fig 3d. Doi: 10.1371/journal.pone.0170908. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab14150 は 11 報の論文で使用されています。
- Zou M et al. Glutathione S-transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer. Oncol Rep 41:989-998 (2019). PubMed: 30431119
- Mayorga ME et al. Role of SDF-1:CXCR4 in Impaired Post-Myocardial Infarction Cardiac Repair in Diabetes. Stem Cells Transl Med 7:115-124 (2018). Functional Studies ; Mouse . PubMed: 29119710
- Stewart SE et al. Transbilayer phospholipid movement facilitates the translocation of annexin across membranes. J Cell Sci 131:N/A (2018). PubMed: 29930080
- Lin Z et al. Inhibition of the CyclinD1 promoter in response to sonic hedgehog signaling pathway transduction is mediated by Gli1. Exp Ther Med 13:307-314 (2017). PubMed: 28123507
- Seyffert M et al. Novel Mutant AAV2 Rep Proteins Support AAV2 Replication without Blocking HSV-1 Helpervirus Replication. PLoS One 12:e0170908 (2017). PubMed: 28125695