Anti-Annexin V/ANXA5 抗体 [EPR3979] (ab108321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3979] to Annexin V/ANXA5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Annexin V/ANXA5 antibody [EPR3979]
Annexin V/ANXA5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3979] to Annexin V/ANXA5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HeLa, JAR, and fetal kidney lysates; Human gastric tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3979 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
|
IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
ターゲット情報
-
機能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
関連疾患
Pregnancy loss, recurrent, 3 -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
-
参照データベース
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
-
別名
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
画像
-
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin V/ANXA5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108321 was shown to specifically react with Annexin V/ANXA5 in wild-type cells as signal was lost in Annexin V/ANXA5 knockout cells. Wild-type and Annexin V/ANXA5 knockout samples were subjected to SDS-PAGE. Ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa -
Immunohistochemical analysis of Annexin V/ANXA5 in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab108321 は 4 報の論文で使用されています。
- Karlsson H et al. Selective radiosensitization by nitazoxanide of quiescent clonogenic colon cancer tumour cells. Oncol Lett 23:123 (2022). PubMed: 35261637
- Spiegelberg D et al. The Novel Anti-cMet Antibody seeMet 12 Potentiates Sorafenib Therapy and Radiotherapy in a Colorectal Cancer Model. Front Oncol 10:1717 (2020). PubMed: 33014851
- Campoy I et al. Exosome-like vesicles in uterine aspirates: a comparison of ultracentrifugation-based isolation protocols. J Transl Med 14:180 (2016). PubMed: 27317346
- Liu B et al. Altered protein expression in gestational diabetes mellitus placentas provides insight into insulin resistance and coagulation/fibrinolysis pathways. PLoS One 7:e44701 (2012). WB, IHC-P ; Human . PubMed: 22970290