Anti-Annexin A1/ANXA1 抗体 (ab88865)
Key features and details
- Mouse polyclonal to Annexin A1/ANXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Annexin A1/ANXA1 antibody
Annexin A1/ANXA1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to Annexin A1/ANXA1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human Annexin A1/ANXA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88865の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IHC-P | ||
ICC/IF |
特記事項 |
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IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method in 1X citrate buffer (pH 6.0) before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
細胞内局在
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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参照データベース
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
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別名
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
画像
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Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml
Lane 1 : Annexin A1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
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ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab88865 は 3 報の論文で使用されています。
- Zhang W et al. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner. Cell Death Dis 12:102 (2021). PubMed: 33473125
- Li L et al. ANXA4 Activates JAK-STAT3 Signaling by Interacting with ANXA1 in Basal-Like Breast Cancer. DNA Cell Biol 39:1649-1656 (2020). PubMed: 32552056
- Marg A et al. Sarcolemmal repair is a slow process and includes EHD2. Traffic 13:1286-94 (2012). PubMed: 22679923