リコンビナント

Anti-Annexin-2/ANXA2 抗体 [D1/274.5] - BSA and Azide free (ab269512)

Submit a review Submit a question
Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
  • Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free
    Annexin-2/ANXA2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2 - BSA and Azide free

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Tissue, cells or virus within Human Annexin-2/ANXA2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Human ovary cancer tissue lysate. HeLa and HUVEC whole cell lysate. ICC/IF: HeLa and MCF7 cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269512 is the carrier-free version of ab269511. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
WB
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 15.46 µg/ml.
WB
Use a concentration of 0.733 µg/ml. Predicted molecular weight: 38 kDa.
特記事項
ICC/IF
Use a concentration of 15.46 µg/ml.
WB
Use a concentration of 0.733 µg/ml. Predicted molecular weight: 38 kDa.
  • 追加情報
    Is unsuitable for IP.

    • ターゲット情報

    • 機能

      Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.

    • 配列類似性

      Belongs to the annexin family.
      Contains 4 annexin repeats.

    • ドメイン

      A pair of annexin repeats may form one binding site for calcium and phospholipid.

    • 翻訳後修飾

      Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
      ISGylated.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Annexin A2 antibody
        • Annexin II antibody
        • Annexin II, heavy chain antibody
        • Annexin-2 antibody
        • ANX 2 antibody
        • ANX2 antibody
        • ANX2L4 antibody
        • ANXA2 antibody
        • ANXA2_HUMAN antibody
        • arylsulfatase B antibody
        • CAL1H antibody
        • Calpactin I heavy chain antibody
        • calpactin I heavy polypeptide (p36) antibody
        • Calpactin I heavy polypeptide antibody
        • Calpactin-1 heavy chain antibody
        • chromobindin 8 antibody
        • Chromobindin-8 antibody
        • Epididymis secretory protein Li 270 antibody
        • HEL S 270 antibody
        • LIP2 antibody
        • Lipocortin II antibody
        • LPC2 antibody
        • LPC2D antibody
        • p36 antibody
        • P36 protein antibody
        • PAP-IV antibody
        • Placental anticoagulant protein IV antibody
        • Protein I antibody
        see all

      画像

      • Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
        Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
        All lanes : Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511) at 0.773 µg/ml

        Lane 1 : Human ovary cancer tissue lysate
        Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
        Lane 3 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

        Predicted band size: 38 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.

        Exposure time: 3 minutes.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

      • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
        Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 9human breast adenocarcinoma cell line) cells labelling Annexin II with ab269511 at 15.46 µg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in MCF7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

      • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
        Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Annexin II with ab269511 at 15.46 µg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

      • Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
        Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269512 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269512 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab269512.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com