Anti-ANKRD57 抗体 (ab99381)
Key features and details
- Rabbit polyclonal to ANKRD57
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ANKRD57 antibody -
製品の詳細
Rabbit polyclonal to ANKRD57 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ANKRD57 aa 475-525. The exact sequence is proprietary.
Database link: Q53LP3 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99381の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 56 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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配列類似性
Belongs to the SOWAH family.
Contains 2 ANK repeats. - Information by UniProt
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参照データベース
- Entrez Gene: 65124 Human
- SwissProt: Q53LP3 Human
- Unigene: 355455 Human
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別名
- 4921515A04Rik antibody
- 9430099J10Rik antibody
- Aankyrin repeat domain 5 antibody
see all
画像
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Western blot - Anti-ANKRD57 antibody (ab99381)All lanes : Anti-ANKRD57 antibody (ab99381) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 56 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ANKRD57 antibody (ab99381)ANKRD57 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab99381 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab99381 at 0.1 µg/ml.
Lane 1: ab99381 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99381 は論文での使用が確認できていません。
