製品の概要

  • 製品名

    Anisomycin, Protein synthesis inhibitor
  • 製品の詳細

    Protein synthesis inhibitor
  • 精製度

    > 98%
  • CAS 番号

    22862-76-6
  • 構造式

    Chemical Structure

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:

  • Croons V  et al. The protein synthesis inhibitor anisomycin induces macrophage apoptosis in rabbit atherosclerotic plaques through p38 mitogen-activated protein kinase. J Pharmacol Exp Ther 329:856-64 (2009). Read more (PubMed: 19286921) »
  • Xiong W  et al. Anisomycin activates p38 MAP kinase to induce LTD in mouse primary visual cortex. Brain Res 1085:68-76 (2006). Read more (PubMed: 16581040) »
  • Cano E  et al. Anisomycin-activated protein kinases p45 and p55 but not mitogen-activated protein kinases ERK-1 and -2 are implicated in the induction of c-fos and c-jun. Mol Cell Biol 14:7352-62 (1994). Read more (PubMed: 7935449) »
See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab120495.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

登録