Anti-Anillin 抗体 - C-terminal (ab154337)
Key features and details
- Rabbit polyclonal to Anillin - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Anillin antibody - C-terminal
Anillin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Anillin - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide, corresponding to a region within C terminal amino acids 1062-1124 of Human Anillin (Uniprot ID: Q9NQW6).
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ポジティブ･コントロール
- 293T and NIH 3T3 whole cell lysates; HeLa cells; Mouse brain, Human U87 xenograft and kidney tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154337の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
組織特異性
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
配列類似性
Contains 1 PH domain. -
発生段階
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
翻訳後修飾
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 54443 Human
- Entrez Gene: 68743 Mouse
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- SwissProt: Q8K298 Mouse
- Unigene: 62180 Human
- Unigene: 282751 Mouse
- Unigene: 453238 Mouse
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別名
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody - C-terminal (ab154337)Immunohistochemical analysis of Paraffin-embedded Human U87 xenograft tissue, labeling Anillin with ab154337 at 1/500 dilution.
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Western blot - Anti-Anillin antibody - C-terminal (ab154337)Anti-Anillin antibody - C-terminal (ab154337) at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 124 kDa
7.5% SDS PAGE -
Western blot - Anti-Anillin antibody - C-terminal (ab154337)Anti-Anillin antibody - C-terminal (ab154337) at 1/500 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 124 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody - C-terminal (ab154337)Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labeling Anillin with ab154337 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody - C-terminal (ab154337)Immunohistochemical analysis of Paraffin-embedded Mouse brain tissue, labeling Anillin with ab154337 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody - C-terminal (ab154337)Immunohistochemical analysis of paraffin-embedded Human Kidney tissue, labeling Anillin with ab154337 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154337 は 3 報の論文で使用されています。
- Castellan RF et al. miR-96 and miR-183 differentially regulate neonatal and adult postinfarct neovascularization. JCI Insight 5:N/A (2020). PubMed: 32544097
- Walton CC et al. Primary neurons can enter M-phase. Sci Rep 9:4594 (2019). PubMed: 30872646
- Zeng S et al. Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma. Sci Rep 7:3151 (2017). IHC-P, IF ; Human . PubMed: 28600503