Anti-Anillin 抗体 (ab99352)
Key features and details
- Rabbit polyclonal to Anillin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Anillin antibody
Anillin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Anillin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Anillin (NP_061155.2)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| IP |
Use a concentration of 2 - 5 µg/ml.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
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IP
Use a concentration of 2 - 5 µg/ml. |
ターゲット情報
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機能
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
組織特異性
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
配列類似性
Contains 1 PH domain. -
発生段階
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
翻訳後修飾
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 54443 Human
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- Unigene: 62180 Human
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別名
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
画像
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Western blot - Anti-Anillin antibody (ab99352)All lanes : Anti-Anillin antibody (ab99352) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Anillin antibody (ab99352)Detection of Human Anillin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99352 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). Anillin was also immunoprecipitated by another rabbit anti-Anillin antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab99352 は 3 報の論文で使用されています。
- Uretmen Kagiali ZC et al. CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Life Sci Alliance 3:N/A (2020). PubMed: 31879279
- S Pedersen R et al. Profiling DNA damage response following mitotic perturbations. Nat Commun 7:13887 (2016). PubMed: 27976684
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416
