Anti-Angiotensin II Type 2 Receptor 抗体 [EPR3876] (ab92445)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Angiotensin II Type 2 Receptor antibody [EPR3876]
Angiotensin II Type 2 Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Angiotensin II Type 2 Receptor aa 300-400 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HepG2, Human heart, Mouse heart, and Rat heart lysates. IP: Mouse heart cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3876 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92445の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation. -
組織特異性
In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 186 Human
- Entrez Gene: 11609 Mouse
- Entrez Gene: 24182 Rat
- Omim: 300034 Human
- SwissProt: P50052 Human
- SwissProt: P35374 Mouse
- SwissProt: Q3US12 Mouse
- SwissProt: P35351 Rat
see all -
別名
- AGTR 2 antibody
- Agtr2 antibody
- AGTR2_HUMAN antibody
see all
画像
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All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
ab92445 (purified ) at 1/20 dilution (0.2ug) immunoprecipitating Angiotensin II Type 2 Receptor in Mouse heart lysate .
Lane 1 (input): Mouse heart lysate 10ug
Lane 2 (+): ab92445 & Mouse heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92445 in Mouse heart lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab92445 は 45 報の論文で使用されています。
- Kim HY et al. Oryeongsan (Wulingsan) ameliorates impaired ANP secretion of atria from spontaneously hypertensive rats. Biomed Pharmacother 146:112433 (2022). PubMed: 35062052
- Garner KL et al. Maternal Nanomaterial Inhalation Exposure: Critical Gestational Period in the Uterine Microcirculation is Angiotensin II Dependent. Cardiovasc Toxicol 22:167-180 (2022). PubMed: 35066857
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Zhang F et al. G-protein-coupled receptor kinase 4 causes renal angiotensin II type 2 receptor dysfunction by increasing its phosphorylation. Clin Sci (Lond) 136:989-1003 (2022). PubMed: 35695067
- Li A et al. The gene knockout of angiotensin II type 1a receptor improves high-fat diet-induced obesity in rat via promoting adipose lipolysis. PLoS One 17:e0267331 (2022). PubMed: 35802723