Anti-Angiopoietin-like 4/ANGPTL4 抗体 (ab2920)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Angiopoietin-like 4/ANGPTL4 antibody
Angiopoietin-like 4/ANGPTL4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4 -
由来種
Rabbit -
特異性
Detects fasting-induced adipose factor (FIAF) from rat adipose tissue. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse Angiopoietin-like 4/ANGPTL4 aa 80-94 (N terminal).
Sequence:CQGPKGKDAPFKDSE
(Peptide available asab4936) -
ポジティブ・コントロール
- Rat adipose tissue extracts.
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特記事項
This product was previously labelled as Angiopoietin-like 4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Adipose tissue is an energy reserve in animals, and in nondomestic species it is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as leptin and Acrp30. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies suggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use a concentration of 2 µg/ml. By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts. |
ターゲット情報
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機能
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
組織特異性
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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参照データベース
- Entrez Gene: 57875 Mouse
- Entrez Gene: 362850 Rat
- SwissProt: Q9Z1P8 Mouse
- SwissProt: Q6TMA8 Rat
- Unigene: 196189 Mouse
- Unigene: 119611 Rat
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別名
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
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画像
データシートおよび資料
参考文献 (5)
ab2920 は 5 報の論文で使用されています。
- Zhou S et al. Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4. Oncol Rep 44:927-938 (2020). PubMed: 32705217
- Plaza A et al. Cholecystokinin is involved in triglyceride fatty acid uptake by rat adipose tissue. J Endocrinol 236:137-150 (2018). PubMed: 29339381
- Rodrigues T et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . PubMed: 28490763
- Villa L et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). PubMed: 21414972
- Koliwad SK et al. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism. J Biol Chem 284:25593-601 (2009). WB ; Mouse . PubMed: 19628874