Anti-Angiopoietin-like 4/ANGPTL4 抗体 (ab196746)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiopoietin-like 4/ANGPTL4 antibody
Angiopoietin-like 4/ANGPTL4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Angiopoietin-like 4/ANGPTL4 (internal sequence).
Database link: Q9BY76 -
ポジティブ・コントロール
- Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869) can be used as a positive control in WB. Mouse skeletal muscle extract; Rat lung and Human esophageal cancer tissues; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/50 - 1/200.
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
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特記事項 |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
ターゲット情報
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機能
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
組織特異性
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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参照データベース
- Entrez Gene: 51129 Human
- Entrez Gene: 57875 Mouse
- Entrez Gene: 362850 Rat
- Omim: 605910 Human
- SwissProt: Q9BY76 Human
- SwissProt: Q9Z1P8 Mouse
- SwissProt: Q6TMA8 Rat
- Unigene: 9613 Human
see all -
別名
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
画像
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Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) at 1/500 dilution + Mouse skeletal muscle extract
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
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Immunofluorescent analysis of A549 cells labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/50 dilution. Blue: DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab196746 は 17 報の論文で使用されています。
- Xiang X et al. Oleic and palmitic acids induce hepatic angiopoietin-like 4 expression predominantly via PPAR-γ in Larimichthys crocea. Br J Nutr 129:1657-1666 (2023). PubMed: 34556193
- Kamaludin Z et al. Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women. Pathophysiology 29:9-23 (2022). PubMed: 35366286
- Zhang K et al. DNA methylation mediated down-regulation of ANGPTL4 promotes colorectal cancer metastasis by activating the ERK pathway. J Cancer 12:5473-5485 (2021). PubMed: 34405010
- Zhu X et al. ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway. Cardiol Res Pract 2021:9935310 (2021). PubMed: 34422410
- Martinho JP et al. Impairment of the angiogenic process may contribute to lower success rate of root canal treatments in diabetes mellitus. Int Endod J N/A:N/A (2021). PubMed: 33999433