Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Umbilical Vein Endothelial Cell)
Permeabilization
Yes - 0.5% Triton X-100 for 20min
Specification
Human Umbilical Vein Endothelial Cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG, Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 23 2017