Anti-Angiopoietin 1 + Angiopoietin 2 抗体 (ab65835)
Key features and details
- Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2
- Suitable for: ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiopoietin 1 + Angiopoietin 2 antibody -
製品の詳細
Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat -
免疫原
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ポジティブ・コントロール
- WB: Rat brain tissue lysate. ICC: PC12 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65835の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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参照データベース
- Entrez Gene: 89805 Rat
- SwissProt: 89807 Rat
- SwissProt: O35460 Rat
- SwissProt: O35462 Rat
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別名
- AGP1 antibody
- AGPT antibody
- AGPT2 antibody
see all
画像
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Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab65835 は 15 報の論文で使用されています。
- Klomjit N et al. Microvascular remodeling and altered angiogenic signaling in human kidneys distal to occlusive atherosclerotic renal artery stenosis. Nephrol Dial Transplant 37:1844-1856 (2022). PubMed: 35451482
- Lun W et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). PubMed: 29977158
- Prapansilp P et al. A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria. Malar J 12:50 (2013). IHC-P ; Human . PubMed: 23383853
- Chaitanya GV et al. Inflammation induces neuro-lymphatic protein expression in multiple sclerosis brain neurovasculature. J Neuroinflammation 10:125 (2013). WB, IHC-P ; Mouse, Human . PubMed: 24124909
- Milton I et al. Age-dependent lethality in novel transgenic mouse models of central nervous system arteriovenous malformations. Stroke 43:1432-5 (2012). IHC-Fr ; Mouse . PubMed: 22328553