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リコンビナントRabMAb

Anti-Androgen Receptor 抗体 [ER179(2)] - ChIP Grade (ab108341)

Reviews (4) Submit a question References (42)
Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
  • Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [ER179(2)] to Androgen Receptor - ChIP Grade
  • Suitable for: ICC/IF, WB, IHC-P, ChIP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

製品の概要

  • 製品名

    Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade
    Androgen Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [ER179(2)] to Androgen Receptor - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, ChIPmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and Rat testis lysates, Human LNCaP, 22Rv1, T47 D and LnCaP whole cell lysates. IHC: Mouse testis, Human testis, breast carcinoma, prostatic carcinoma and normal testis tissue. ICC/IF: Human LnCaP cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108341の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/500.

For unpurified use at 1/100 - 1/250.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500. 

Perform heat mediated antigen retrieval with citrate buffer (pH 6.0) or Tris-EDTA buffer (pH9.0) before commencing with IHC staining protocol.
ChIP
Use at an assay dependent concentration.

PubMed: 23817021
特記事項
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500. 

Perform heat mediated antigen retrieval with citrate buffer (pH 6.0) or Tris-EDTA buffer (pH9.0) before commencing with IHC staining protocol.
ChIP
Use at an assay dependent concentration.

PubMed: 23817021
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

  • 組織特異性

    Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).

  • 関連疾患

    Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.

  • 翻訳後修飾

    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • 細胞内局在

    Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.

  • 別名

    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

画像

  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution

    Lane 1 : Mouse testis lysates
    Lane 2 : Rat testis lysates
    Lane 3 : Mouse liver lysates
    Lane 4 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa


    Exposure time: 20 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Observed MW; 110 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Immunohistochemistry (paraffin-embedded sections) analysis of mouse testis tissue labelling Androgen with ab108341 at 0.12 µg/mL for 30mins at room temperature. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/4000). Counterstained with hematoxylin. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 4 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST

    The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.

    We recommend you to try both RIPA and 1% SDS Hot lysis preparation methods to get desired bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling Androgen receptor with Purified ab108341 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Chromatin was prepared from LNCaP cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab108341 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are commercial primers from Paper (PMID: 25802280)
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling Androgen receptor with Purified ab108341 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution (purified) + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution (unpurified)

    Lane 1 : LnCAP whole cell lysate (positive control)
    Lane 2 : PC3 whole cell lysate (negative control)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab108341 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108341 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341, at 1/100, staining Androgen Receptor in LnCaP cells by Immunofluorescence.

  • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution (unpurified)

    Lane 1 : T47 D cell lysate
    Lane 2 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 99 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostatic adenocarcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostate tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341 showing positive staining in Normal testis tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341 showing positive staining in Prostatic carcinoma T3 tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified  ab108341 showing positive staining in Breast carcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

    Unpurified ab108341 showing negative staining in Normal liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
    Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

参考文献 (42)

ab108341 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108341 は 42 報の論文で使用されています。

  • He Y  et al. A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Nat Commun 12:1521 (2021). PubMed: 33750801
  • Wang X  et al. Kaempferol inhibits benign prostatic hyperplasia by resisting the action of androgen. Eur J Pharmacol 907:174251 (2021). PubMed: 34129879
  • Behairy A  et al. Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role. Front Pharmacol 12:651497 (2021). PubMed: 33986679
  • Wang C  et al. Expression of G3BP1 in benign and malignant human prostate tissues. Transl Androl Urol 10:1665-1675 (2021). PubMed: 33968655
  • Strittmatter BG  et al. Ras/ERK and PI3K/AKT signaling differentially regulate oncogenic ERG mediated transcription in prostate cells. PLoS Genet 17:e1009708 (2021). PubMed: 34314419
View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Oct 07 2021

ELISA abreview for Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Human Cell (HEK293T cells)
Specification
HEK293T cells
Blocking step
Superblock (Thermo Fisher) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Type
Sandwich (Detection)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 29 2021

Western blot abreview for Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Western blot abreview for Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
LNCaP cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

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