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RabMAb

Anti-Androgen Receptor 抗体 [ER179(2)] - ChIP Grade (ab108341)

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製品の概要

  • 製品名

    Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade
    Androgen Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [ER179(2)] to Androgen Receptor - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, ChIPmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Androgen Receptor aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • T47-D and LnCaP cell lysates. Paraffin-embedded human prostatic adenocarcinoma tissue. Paraffin-embedded human prostate tissue. Human testis

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500. 

Perform heat mediated antigen retrieval with citrate buffer (pH 6.0) or Tris-EDTA buffer (pH9.0) before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.

PubMed: 23817021
  • 追加情報
    Is unsuitable for Flow Cyt or IP.

    • ターゲット情報

    • 機能

      Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
      Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

    • 組織特異性

      Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).

    • 関連疾患

      Androgen insensitivity syndrome
      Spinal and bulbar muscular atrophy X-linked 1
      Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
      Androgen insensitivity, partial

    • 配列類似性

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • ドメイン

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.

    • 翻訳後修飾

      Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
      Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

    • 細胞内局在

      Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
    • Information by UniProt

    • 参照データベース

      • 製品の状態

        There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.

      • 別名

        • AIS antibody
        • ANDR_HUMAN antibody
        • Androgen nuclear receptor variant 2 antibody
        • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
        • Androgen receptor antibody
        • androgen receptor splice variant 4b antibody
        • AR antibody
        • AR8 antibody
        • DHTR antibody
        • Dihydro testosterone receptor antibody
        • Dihydrotestosterone receptor (DHTR) antibody
        • Dihydrotestosterone receptor antibody
        • HUMARA antibody
        • HYSP1 antibody
        • KD antibody
        • Kennedy disease (KD) antibody
        • NR3C4 antibody
        • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
        • Nuclear receptor subfamily 3 group C member 4 antibody
        • SBMA antibody
        • SMAX1 antibody
        • Spinal and bulbar muscular atrophy (SBMA) antibody
        • Spinal and bulbar muscular atrophy antibody
        • Testicular Feminization (TFM) antibody
        • TFM antibody
        see all

      画像

      • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution

        Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
        Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
        Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
        Lane 4 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Predicted band size: 99 kDa
        Observed band size: 120 kDa
        why is the actual band size different from the predicted?


        Exposure time: 20 seconds


        Blocking/Diluting buffer: 5% NFDM/TBST

        The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.

        We recommend you to try both RIPA and 1% SDS Hot lysis preparation methods to get desired bands.

      • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling Androgen receptor with Purified ab108341 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        ChIP - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Chromatin was prepared from LNCaP cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
        The ChIP was performed with 25 µg of chromatin, 5 µg of ab108341 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
        Primers and probes are commercial primers from Paper (PMID: 25802280)
        *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling Androgen receptor with Purified ab108341 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/5000 dilution (purified) + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 99 kDa



        Blocking and diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution (unpurified)

        Lane 1 : LnCAP whole cell lysate (positive control)
        Lane 2 : PC3 whole cell lysate (negative control)

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 99 kDa
        Observed band size: 120 kDa why is the actual band size different from the predicted?



        Lanes 1 - 2: Merged signal (red and green). Green - ab108341 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.

        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108341 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

      • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341, at 1/100, staining Androgen Receptor in LnCaP cells by Immunofluorescence.

      • Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        All lanes : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341) at 1/1000 dilution (unpurified)

        Lane 1 : T47 D cell lysate
        Lane 2 : LnCaP cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 99 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostatic adenocarcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostate tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341 showing positive staining in Normal testis tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341 showing positive staining in Prostatic carcinoma T3 tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified  ab108341 showing positive staining in Breast carcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (ab108341)

        Unpurified ab108341 showing negative staining in Normal liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      参考文献

      ab108341 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab108341 は 19 報の論文で使用されています。

      • Lee E  et al. GREB1 amplifies androgen receptor output in human prostate cancer and contributes to antiandrogen resistance. Elife 8:N/A (2019). PubMed: 30644358
      • Berger A  et al. N-Myc-mediated epigenetic reprogramming drives lineage plasticity in advanced prostate cancer. J Clin Invest 130:3924-3940 (2019). PubMed: 31260412
      • Bai Y  et al. RUNX2 overexpression and PTEN haploinsufficiency cooperate to promote CXCR7 expression and cellular trafficking, AKT hyperactivation and prostate tumorigenesis. Theranostics 9:3459-3475 (2019). PubMed: 31281490
      • Liao Y  et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018). Human . PubMed: 29353883
      • Puca L  et al. Patient derived organoids to model rare prostate cancer phenotypes. Nat Commun 9:2404 (2018). PubMed: 29921838
      View all Publications for this product

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