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Synthetic peptide within Human Androgen Receptor aa 300-400 (N terminal). The exact sequence is proprietary.
Database link: P10275
Our Abpromise guarantee covers the use of ab74272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24466341|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21430025|
ab74272 used in ChIP application.Whole cell lysate from human LNCaP cells was used.Cross linking step (X-ChIP) for 10 minutes in 1% formaldehyde.Taqman detection step.Positive control was PSA enhancer.Negative control region, no antibody control, and in the absence of ligand.25 µl undiluted ab74272 per IP was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma labelling Androgen Receptor with ab74272 at a dilution of 1/200 for 30mins at room temperature.
ab74272 staining Androgen Receptor in mouse neuroblastoma cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 594-conjugated goat anti-rabbit polyclonal, diluted 1/400.
ab74272 staining Androgen Receptor in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA and blocked with 5% BSA for 45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
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