Anti-Androgen Receptor 抗体 [AR 441] (ab9474)
Key features and details
- Mouse monoclonal [AR 441] to Androgen Receptor
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Androgen Receptor antibody [AR 441]
Androgen Receptor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AR 441] to Androgen Receptor -
由来種
Mouse -
特異性
This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors. -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Macaque monkey
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免疫原
Synthetic peptide corresponding to Human Androgen Receptor aa 302-318.
Sequence:STEDTAEYSPFKGGYTK
Database link: P10275 -
ポジティブ･コントロール
- Prostate Carcinoma.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
purified immunoglobulin fraction. -
ポリ/モノ
モノクローナル -
クローン名
AR 441 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. -
組織特異性
Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107). -
関連疾患
Androgen insensitivity syndrome
Spinal and bulbar muscular atrophy X-linked 1
Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
Androgen insensitivity, partial -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain. -
翻訳後修飾
Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
細胞内局在
Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. - Information by UniProt
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参照データベース
- Entrez Gene: 367 Human
- Omim: 313700 Human
- SwissProt: P10275 Human
- Unigene: 76704 Human
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製品の状態
There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45. -
別名
- AIS antibody
- ANDR_HUMAN antibody
- Androgen nuclear receptor variant 2 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (ab9474)](https://www.abcam.co.jp/ps/products/9/ab9474/Images/ab9474-17032-anti-androgen-receptor-antibody-ar-441-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (ab9474)ab9474 staining human prostate by IHC-P.
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![Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)](https://www.abcam.co.jp/ps/products/9/ab9474/Images/ab9474-17030-anti-androgen-receptor-antibody-ar-441-flow-cytometry.jpg)
Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)](https://www.abcam.co.jp/ps/products/9/ab9474/Images/ab9474-17029-anti-androgen-receptor-antibody-ar-441-flow-cytometry.jpg)
Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
プロトコール
参考文献 (31)
ab9474 は 31 報の論文で使用されています。
- Younas K et al. Delayed endometrial decidualisation in polycystic ovary syndrome; the role of AR-MAGEA11. J Mol Med (Berl) 97:1315-1327 (2019). PubMed: 31256208
- Cao L et al. A high AR:ERa or PDEF:ERa ratio predicts a sub-optimal response to tamoxifen therapy in ERa-positive breast cancer. Cancer Chemother Pharmacol 84:609-620 (2019). PubMed: 31297554
- Liu X et al. Androgen receptor promotes oral squamous cell carcinoma cell migration by increasing EGFR phosphorylation. Onco Targets Ther 12:4245-4252 (2019). PubMed: 31239703
- Wang R et al. Androgen Receptor Promotes Gastric Carcinogenesis via Upregulating Cell Cycle-Related Kinase Expression. J Cancer 10:4178-4188 (2019). PubMed: 31413736
- Giatromanolaki A et al. CYP17A1 and Androgen-Receptor Expression in Prostate Carcinoma Tissues and Cancer Cell Lines. Curr Urol 13:157-165 (2019). PubMed: 31933595
