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  1. Link

    androgen-receptor-antibody-ab19066.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Testosterone
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Anti-Androgen Receptor 抗体 (ab19066)

  • Datasheet
Submit a review Q&A (1)References (3)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)
  • Western blot - Anti-Androgen Receptor antibody (ab19066)

Key features and details

  • Goat polyclonal to Androgen Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Androgen Receptor antibody
    Androgen Receptor 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to Androgen Receptor
  • 由来種

    Goat
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide:

    EVQLGLGRVYPRPPSC

    , corresponding to N terminal amino acids 2-16 of Human Androgen Receptor
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • Human Brain and Human Heart lysates.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Testosterone
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Testosterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Other
    • Developmental Biology
    • Reproduction
    • Hormones
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Androgen Receptor protein (His tag) (ab82124)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab19066の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

A 1 hour primary incubation is recommended for this product.

IHC-P
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

A 1 hour primary incubation is recommended for this product.

IHC-P
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • 組織特異性

    Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • 関連疾患

    Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • 翻訳後修飾

    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 細胞内局在

    Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Target information above from: UniProt accession P10275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 747460 Chimpanzee
    • Entrez Gene: 280675 Cow
    • Entrez Gene: 403588 Dog
    • Entrez Gene: 367 Human
    • Entrez Gene: 11835 Mouse
    • Entrez Gene: 397582 Pig
    • Entrez Gene: 24208 Rat
    • Omim: 313700 Human
    • SwissProt: O97775 Chimpanzee
    • SwissProt: Q9TT90 Dog
    • SwissProt: P10275 Human
    • SwissProt: P19091 Mouse
    • SwissProt: Q9GKL7 Pig
    • SwissProt: P15207 Rat
    • Unigene: 76704 Human
    • Unigene: 39005 Mouse
    • Unigene: 394224 Mouse
    • Unigene: 439657 Mouse
    • Unigene: 9813 Rat
    see all
  • 製品の状態

    There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
  • 別名

    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)

    ab19066 (2µg/ml) staining of paraffin embedded Human Prostate following steamed antigen retrieval with citrate buffer pH 6 and  AP-staining shows nuclear staining in the secretory cells of the gland.

  • Western blot - Anti-Androgen Receptor antibody (ab19066)
    Western blot - Anti-Androgen Receptor antibody (ab19066)

    ab19066 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) approx. 99kDa.  Primary incubation was 1 hour.  Detected by chemiluminescence.

    ab19066 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) approx. 99kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (3)

ab19066 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab19066 は 3 報の論文で使用されています。

  • Jin M  et al. The effects of neuromedin S on the hypothalamic-pituitary-testicular axis in male pigs in vitro. Gen Comp Endocrinol N/A:N/A (2019). PubMed: 30981702
  • McWhorter ES  et al. LIN28B regulates androgen receptor in human trophoblast cells through Let-7c. Mol Reprod Dev 86:1086-1093 (2019). PubMed: 31215730
  • Titus MA  et al. Dominant-negative androgen receptor inhibition of intracrine androgen-dependent growth of castration-recurrent prostate cancer. PLoS One 7:e30192 (2012). WB ; Human . PubMed: 22272301

レビューと Q&A

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レビューを送る 質問を送る

Question

BATCH NUMBER 380693 ORDER NUMBER 314211 DESCRIPTION OF THE PROBLEM No band at all. SAMPLE Protein extracted from LNCaP cells PRIMARY ANTIBODY Abcam/goat polyclonal to Androgen Receptor (ab19066)/1% non fat dry milk in TBST/ 1:250/ incubate overnight at 4°C/ wash with TBST once for 15 minutes and 4 times for 5 mins. DETECTION METHOD autorad film POSITIVE AND NEGATIVE CONTROLS USED None used. Did not have such controls at the time the experiment was run. ANTIBODY STORAGE CONDITIONS aliquoted and stored at -20°C SAMPLE PREPARATION RIPA buffer/EDTA-free protease inhibitor cocktail/heated sample for 5 minutes at 95°C AMOUNT OF PROTEIN LOADED Total protein extracted was about 100 ug/ml and 40 ul was loaded into gel. ELECTROPHORESIS/GEL CONDITIONS Reducing gel, 7.5% Tris-HCl ready gel TRANSFER AND BLOCKING CONDITIONS Transfer Buffer (25mM Tris, 192 mM glycine, 20% v/v methanol,pH 8.3)/ 100 volts for 120 mins. 5% non-fat dry milk was used as blocking buffer, incubation period was 1 hr. SECONDARY ANTIBODY Abcam/Rabbit polyclonal to Goat IgG - H&L (HRP) (ab6741)/1% non fat dry milk in TBST/ 1:5000/ incubate for 2 hrs at room temperature/ wash with TBST once for 15 minutes and 4 times for 5 mins. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Protein concentration, primary antibody dilution (1:100 - 1:1000) and incubation time (1 hr at room temperature to overnight at 4°C),secondary antibody dilution (1:4000 - 1:7000) and incubation time( 1 - 2 hrs at room tempertature) and auto rad film exposure time (10 sec to 2 hrs) ADDITIONAL NOTES I have run western blot on protein extracted from LNCaP cells several time with no results. I do not get any bands at all. I have optimized what I believe are the critical stages of the process e.g. protein concentration, primary antibody dilution and incubation times, secondary antibody dilution and incubation times and the wash steps for each stage. As such, I am beginning to wonder whether there might be a problem with the antibody itself. Please advise. Also could you kindly send me a replacement that works? Thanks

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Abcam community

Verified customer

Asked on Feb 11 2008

Answer

Thank you for your protocol details, I am very sorry to hear you are experiencing problems with ab19066. I think your protocol is really good and your optimisation steps very detailed and thorough. I have noticed two points which may be the reasons why you are not seeing any bands currently: -the amount you are loading is only 4ug/lane according to my calculations. We recommend to load 20-40ug; I would recommend to increase the amount to 40ug. -the secondary antibody may be the problem: could you test the primary with another secondary antibody. We'll happily offer a replacement vial if the secondary antibody is at fault, under our 4 months Abpromise. I hope these suggestions will resolve the problem; if you still have problems when loading more antibody and the secondary antibody is not at fault please contact us again and we will arrange a replacement vial of ab19066.

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Abcam Scientific Support

Answered on Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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