Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV-2 cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
40 µg
Treatment
1 μg/mL LPS for 24h
Specification
BV-2 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: mouse anti-rabbit IgG-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
4 second(s)
Bands
Specific: 87 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Ji Hye Han
Verified customer
投稿 Jul 31 2018