Anti-Amylin/DAP 抗体 [EPR22556-138] (ab254259)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22556-138] to Amylin/DAP
- Suitable for: Flow Cyt (Intra), IHC-P, IHC-Fr, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Amylin/DAP antibody [EPR22556-138]
Amylin/DAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22556-138] to Amylin/DAP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, IHC-Fr, ICC/IF, IPmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- IHC-P: Human, mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue. ICC/IF: Beta-TC-6 cells. Flow Cyt (intra): Beta-TC-6 cells. IP: Beta-TC-6 whole cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22556-138 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab254259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
|
|
IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
|
ICC/IF |
1/1000.
|
|
IP |
1/30.
|
特記事項 |
---|
Flow Cyt (Intra)
1/500. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
ICC/IF
1/1000. |
IP
1/30. |
ターゲット情報
-
機能
Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. -
配列類似性
Belongs to the calcitonin family. -
ドメイン
The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation. -
翻訳後修飾
Amyloid fibrils are degraded by IDE. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 3375 Human
- Entrez Gene: 15874 Mouse
- Entrez Gene: 24476 Rat
- Omim: 147940 Human
- SwissProt: P10997 Human
- SwissProt: P12968 Mouse
- SwissProt: P12969 Rat
- Unigene: 46835 Human
see all -
別名
- Amylin antibody
- DAP antibody
- Diabetes associated peptide antibody
see all
画像
-
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in human pancreas (PMID: 30014749). The section was incubated with ab254259 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in mouse pancreas (PMID: 30014749). The section was incubated with ab254259 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Amylin/DAP with ab254259 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the islet in rat pancreas (PMID: 30014749). The section was incubated with ab254259 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling Amylin/DAP with ab254259 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on beta cells of mouse pancreatic islets (PMID: 14656003) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas tissue labeling Amylin/DAP with ab254259 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on beta cells of rat pancreatic islets (PMID: 14656003) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling Amylin/DAP with ab254259 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Beta-TC-6 cell line. Negative control: NIH/3T3 (PMID:15802374). The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.
-
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / Beta-TC-6 (Mouse pancreas insulinoma beta cell, Right) cell lines labeling Amylin/DAP with ab254259 at 1/500 (red) compared with a Rabbit monoclonal IgG isitype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Negative control: NIH/3T3 (PMID:15802374).
-
Amylin/DAP was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab254259 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254259 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input).
Lane 2: ab254259 IP in Beta-TC-6 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254259 in Beta-TC-6 whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab254259 は 1 報の論文で使用されています。
- Perez-Frances M et al. Pancreatic Ppy-expressing ?-cells display mixed phenotypic traits and the adaptive plasticity to engage insulin production. Nat Commun 12:4458 (2021). PubMed: 34294685