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Anti-Amylin/DAP 抗体 (ab18018)

Submit a review Q&A (2)

Key features and details

  • Rabbit polyclonal to Amylin/DAP
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Amylin/DAP antibody [EPR22556-138] (ab254259)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Amylin/DAP antibody
    Amylin/DAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Amylin/DAP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human Amylin/DAP aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P10997

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18018の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

  • 配列類似性

    Belongs to the calcitonin family.

  • ドメイン

    The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.

  • 翻訳後修飾

    Amyloid fibrils are degraded by IDE.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Amylin antibody
      • DAP antibody
      • Diabetes associated peptide antibody
      • Diabetes-associated peptide antibody
      • IAP antibody
      • IAPP antibody
      • IAPP_HUMAN antibody
      • Insulinoma amyloid peptide antibody
      • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
      • Islet amyloid polypeptide antibody
      see all

    参考文献 (0)

    ab18018 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18018 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Hi,

    I am trying to detect Human amylin/ Human Amylin Oligomer expressed in tissues of Human amylin transgenic mice via Western blot and immunohistochemistry. I have found 4 antibodies from your website that may be suitable for the work.

    -Anti-Amylin antibody (ab55411)

    -Anti-Amylin antibody, prediluted (ab15128)

    -Anti-Amylin antibody (ab18018)

    -Anti-Amylin antibody (ab15125)

    I am just wondering if you could recommend one or two that maybe best suited for work I planned to do? The antibody should be specifically for human and does not cross react with mouse or rat. Thanks.


    Kind Regards

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.
    Concerning ab18018 - there is significant sequence homology between human amylin and that of mouse and rat, but this antibody has only been tested with human samples.
    Unfortunately, we do not have any experimental data regarding the cross-reactivity of ab15125 and ab15128 and ab55411 with mouse or rat samples too.
    I compared the immunogen for ab15125 with that of the mouse and rat Amylin proteins and the percentage homology for both is 100%. Based on that information ab15125 is most likely not suitable for your application.
    Please do not hesitate to contact me with additional questions or concerns.

    Read More

    Question

    1)Antibody code ab18018 2)Batch number 96335 3)Purchase order number or preferably Abcam order number our purchase order number was 7150 but I think you may put six zeros before that. It was ordered via the Babraham Institute stores. 4)General Information Antibody storage conditions (temperature/reconstitution etc) Arrived at ambient temperature, stored at 4oC overnight, used the following day and the day after. 5)Description of the problem (high background, no signal, non-specific color development, poor standard curve etc.) No signal or weak signal Type of ELISA (Direct ELISA/Indirect ELISA/Sandwich ELISA etc.) Indirect 6)Sample (Species/Cell type/Cell line etc.) Synthetic peptides 7)Coating well (Buffer/Concentration of the coating material etc.) Bicarbonate buffer. Peptide concentration 0.1ng-50ug/well coated O/N 4oC 8)Blocking conditions (Buffer/time period, Blocking agent etc.) 5% Milk/PBS/0.05%Tween 9)Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Ab18018 (the antibody in question), in blocking buffer above at 1:1000-1:100000, 1hr RT Washed in PBS/0.05% Tween x5 10)Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Goat anti-rabbit IgG whole molecule HRP Sigma in blocking buffer Wash as above x5 11)Detection method (Substrate/Diluent etc.) TMB 12)Positive and negative controls used (please specify) Mouse R10/99 mAb and our own polyclonal rabbit anti-human Ab both work extremely well. 13)Optimization attempts (problem solving) How many times have you tried the ELISA? Twice 14)Have you run a "No Primary" control? Yes 15)Do you obtain the same results every time? Yes. 16)What steps have you altered? Concentration of coating material, peptide species and length, concentration of the antibody, 17)Additional Notes Two experiments were carried out by different scientists on different days. Used different batches of various peptides for coating. Please don't hesitate to contact me again if you require more information.

    Read More
    Answer

    Thank you very much for answering our questions. We would like to recommend the following modifications to your protocol: incubate the primary antibody overnight at 4C and try a 1-3% BSA block instead of milk. As the antibody is polyclonal it will recognise a variety of sites on the protein and this maybe less specific than ab11022 and hence require longer incubation times. You mention "synthetic peptides" as your samples, are those human sequences (the antibody has only been tested against human amylin)? We would also suggest doing a dot blot to check that the antibody binds to the peptides. Please do not hesitate to contact us again if you need further advice and be assured that should you still have problems in the recommended conditions we will offer you a replacement vial.

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