製品名Amthamine dihydrobromide, H2 agonist
製品の詳細Selective H2 agonist
生理活性の詳細Selective H2 agonist (pKi = 5.2). Weak antagonist of H3 and displays no activity at H1. Increases gastric acid secretion. Additionally increases blood pressure.
保存方法Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab78079 staining PKA R2 (phospho S96) in HepG2 cells treated with amthamine dihydrobromide (ab120778), by ICC/IF. Increase of PKA R2 (phospho S96) expression correlates with increased concentration of amthamine dihydrobromide, as described in literature.
The cells were incubated at 37°C for 60 minutes in media containing different concentrations of ab120778 (amthamine dihydrobromide) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab78079 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Lim HD et al. Evaluation of histamine H1-, H2-, and H3-receptor ligands at the human histamine H4 receptor: identification of 4-methylhistamine as the first potent and selective H4 receptor agonist. J Pharmacol Exp Ther 314:1310-21 (2005). Read more (PubMed: 15947036) »
- Poli E et al. In vitro cardiac pharmacology of the new histamine H2-receptor agonist amthamine: comparisons with histamine and dimaprit. Agents Actions 40:44-9 (1993). Read more (PubMed: 8147269) »
- Coruzzi G et al. The new potent and selective histamine H2 receptor agonist amthamine as a tool to study gastric secretion. Naunyn Schmiedebergs Arch Pharmacol 348:77-81 (1993). Read more (PubMed: 8377843) »