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  1. Link

    ampk-alpha-2-phospho-s491-antibody-epr3052-ab109402.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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リコンビナントRabMAb

Anti-AMPK alpha 2 (phospho S491) 抗体 [EPR3052] (ab109402)

  • Datasheet
Reviews (1) Submit a question References (3)

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Abpromise

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Western blot - Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)
  • Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3052] to AMPK alpha 2 (phospho S491)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human AMPK alpha 2 protein (ab159189)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052]
    AMPK alpha 2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR3052] to AMPK alpha 2 (phospho S491)
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR3052
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

関連製品

  • Alternative Versions

    • Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] - BSA and Azide free (ab232519)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 2 protein (ab159189)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109402の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
特記事項
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

ターゲット情報

  • 機能

    Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization (PubMed:25687571). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1.
  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • ドメイン

    The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.
  • 翻訳後修飾

    Ubiquitinated.
    Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1).
  • 細胞内局在

    Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
  • Target information above from: UniProt accession P54646 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5563 Human
    • Entrez Gene: 108079 Mouse
    • Entrez Gene: 78975 Rat
    • Omim: 600497 Human
    • SwissProt: P54646 Human
    • SwissProt: Q8BRK8 Mouse
    • SwissProt: Q09137 Rat
    • Unigene: 437039 Human
    • Unigene: 48638 Mouse
    • Unigene: 64583 Rat
    see all
  • 別名

    • 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
    • AAPK2_HUMAN antibody
    • ACACA kinase antibody
    • Acetyl CoA carboxylase kinase antibody
    • Acetyl-CoA carboxylase kinase antibody
    • AMPK alpha 2 chain antibody
    • AMPK subunit alpha-2 antibody
    • AMPK2 antibody
    • AMPKa2 antibody
    • AMPKalpha2 antibody
    • HMGCR kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • Hydroxymethylglutaryl-CoA reductase kinase antibody
    • PRKAA antibody
    • PRKAA2 antibody
    • Protein kinase AMP activated alpha 2 catalytic subunit antibody
    • Protein kinase AMP activated catalytic subunit alpha 2 antibody
    see all

画像

  • Western blot - Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)
    Western blot - Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)
    All lanes : Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402) at 1/1000 dilution (Purified)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa

  • Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)
    Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402)

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (3)

ab109402 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109402 は 3 報の論文で使用されています。

  • Mou SQ  et al. Liquiritin Attenuates Lipopolysaccharides-Induced Cardiomyocyte Injury via an AMP-Activated Protein Kinase-Dependent Signaling Pathway. Front Pharmacol 12:648688 (2021). PubMed: 34054527
  • Chen X  et al. LncRNA NEAT1 promotes hepatic lipid accumulation via regulating miR-146a-5p/ROCK1 in nonalcoholic fatty liver disease. Life Sci 235:116829 (2019). PubMed: 31484042
  • Zeng Q  et al. Electroacupuncture Preconditioning Improves Myocardial Infarction Injury via Enhancing AMPK-Dependent Autophagy in Rats. Biomed Res Int 2018:1238175 (2018). PubMed: 30175112

レビューと Q&A

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Western blot abreview for Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Monkey Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 19 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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