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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-AMPK alpha 1 抗体 [Y365] (ab32047)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (82)

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Abpromise

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Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Anti-AMPK alpha 1 antibody [Y365] (ab32047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y365] to AMPK alpha 1
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

関連製品

製品の概要

  • 製品名

    Anti-AMPK alpha 1 antibody [Y365]
    AMPK alpha 1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [Y365] to AMPK alpha 1
  • 由来種

    Rabbit
  • 特異性

    This antibody is specific for human AMPK alpha 1. This antibody shows low affinity on mouse and rat samples.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human AMPK alpha 1 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13131

  • ポジティブ・コントロール

    • WB: HeLa, HepG2, C6, NIH/3T3 and MCF-7 cell lysate. Mouse liver, brain, retina, and skeletal muscle tissue lysates. IHC-P: Human cervical carcinoma and lung carcinoma tissues. ICC/IF: MCF-7 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate (ab150035).
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    Y365
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

関連製品

  • Alternative Versions

    • Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)
    • Alexa Fluor® 647 Anti-AMPK alpha 1 antibody [Y365] (ab214678)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 1 protein (ab202156)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Mouse
Rat
Human
All applications
African green monkey
アプリケーション Abreviews 特記事項
ICC/IF
1/250.
Flow Cyt
1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (5)
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.

This antibody shows low affinity on mouse and rat samples.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Mouse and rat species are recommended by WB application. 

IP
1/40 - 1/50.
特記事項
ICC/IF
1/250.
Flow Cyt
1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.

This antibody shows low affinity on mouse and rat samples.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Mouse and rat species are recommended by WB application. 

IP
1/40 - 1/50.

ターゲット情報

  • 機能

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13131 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5562 Human
    • Entrez Gene: 105787 Mouse
    • Entrez Gene: 65248 Rat
    • Omim: 602739 Human
    • SwissProt: Q13131 Human
    • SwissProt: Q5EG47 Mouse
    • SwissProt: P54645 Rat
    • Unigene: 43322 Human
    • Unigene: 207004 Mouse
    • Unigene: 87789 Rat
    see all
  • 別名

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

画像

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: AMPK alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32047 observed at 63 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab32047 was shown to specifically react with AMPK alpha in wild-type HAP1 cells. No band was observed when AMPK alpha knockout samples were examined. Wild-type and AMPK alpha knockout samples were subjected to SDS-PAGE. ab32047 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling AMPK alpha 1 with purified ab32047 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 before commencing with IHC staining protocol. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. 

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling AMPK alpha 1 with purified ab32047 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Flow Cytometry analysis of HeLa cells labelling AMPK alpha 1 with purified ab32047 at 1/150 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    ab32047 (purified) at 1/40 immunoprecipitating AMPK alpha 1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab32047 + HeLa whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32047 in HeLa whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse retina tissue lysate
    Lane 7 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 40 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This antibody shows low affinity on mouse samples.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 180 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This antibody shows low affinity on mouse and rat samples.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/2000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.
    This antibody shows low affinity on mouse and rat samples.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analaysis of human cervical carcinoma tissue labelling AMPK alpha 1 with unpurified ab32047 at a dilution of 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Overlay histogram showing HeLa cells stained with unpurified ab32047 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab32047, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Anti-AMPK alpha 1 antibody [Y365] (ab32047)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (82)

    ab32047 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32047 は 82 報の論文で使用されています。

    • Hawley SA  et al. Mechanism of Activation of AMPK by Cordycepin. Cell Chem Biol 27:214-222.e4 (2020). PubMed: 31991096
    • Gu X  et al. Effects of Huang-Lian-Jie-Du Decoction on Oxidative Stress and AMPK-SIRT1 Pathway in Alzheimer's Disease Rat. Evid Based Complement Alternat Med 2020:6212907 (2020). PubMed: 31976005
    • Ji R  et al. Electric field down-regulates CD9 to promote keratinocytes migration through AMPK pathway. Int J Med Sci 17:865-873 (2020). PubMed: 32308539
    • Wu H  et al. MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1a/CEBPB axis to promote stemness and chemo-resistance of gastric cancer. Cell Death Dis 11:233 (2020). PubMed: 32300102
    • Gong H  et al. Nrf2-SHP Cascade-Mediated STAT3 Inactivation Contributes to AMPK-Driven Protection Against Endotoxic Inflammation. Front Immunol 11:414 (2020). PubMed: 32210977
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Gastric Cancer Cell Line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    Gastric Cancer Cell Line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 13 2020

    Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse macrophage cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    3 µg
    Specification
    Mouse macrophage cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2020

    Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Muscle fibers)
    Gel Running Conditions
    Reduced Denaturing (8% acrylamide)
    Loading amount
    50 µg
    Specification
    Muscle fibers
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Irene Tobias

    Verified customer

    投稿 Mar 21 2018

    Immunohistochemistry free floating abreview for Anti-AMPK alpha 1 antibody [Y365]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain slide)
    Specification
    Brain slide
    Read More

    Delphine Franssen

    Verified customer

    投稿 May 17 2017

    Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bos taurus Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-15% Gradient)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Julie Kim

    Verified customer

    投稿 May 05 2017

    Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human HepG2 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    human HepG2 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 05 2016

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