Anti-AMPK alpha 1 抗体 (ab3759)
Key features and details
- Rabbit polyclonal to AMPK alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AMPK alpha 1 antibody
AMPK alpha 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AMPK alpha 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Cow, Human
交差が予測される動物種: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus
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免疫原
Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 63 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5562 Human
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: P54645 Rat
- Unigene: 43322 Human
- Unigene: 87789 Rat
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別名
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
画像
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Western blot - Anti-AMPK alpha 1 antibody (ab3759)
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).
Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
参考文献 (23)
ab3759 は 23 報の論文で使用されています。
- Li T et al. MicroRNA-206 inhibition and activation of the AMPK/Nampt signalling pathway enhance sevoflurane post-conditioning-induced amelioration of myocardial ischaemia/reperfusion injury. J Drug Target 28:80-91 (2020). PubMed: 31092059
- Huang Q et al. Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function. Exp Ther Med 17:1863-1869 (2019). PubMed: 30783461
- Qin Q et al. Ginsenoside Rg1 ameliorates cardiac oxidative stress and inflammation in streptozotocin-induced diabetic rats. Diabetes Metab Syndr Obes 12:1091-1103 (2019). PubMed: 31372019
- Wang BZ et al. AMPK Signaling Regulates the Age-Related Decline of Hippocampal Neurogenesis. Aging Dis 10:1058-1074 (2019). PubMed: 31595203
- Vistro WA et al. In Vivo Autophagy Up-Regulation of Small Intestine Enterocytes in Chinese Soft-Shelled Turtles during Hibernation. Biomolecules 9:N/A (2019). PubMed: 31683886
